Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3693

Full Length Research Paper

Isolation of a novel piperidide from Achillea ‘Moonshine’ using bioactivity guided fractionation for the treatment of acne

Rahul M. Shah
  • Rahul M. Shah
  • Department of Chemistry and Biochemistry, University of the Sciences, Philadelphia, USA.
  • Google Scholar
Tejal Patel
  • Tejal Patel
  • Department of Pharmacognosy, L.M. College of Pharmacy, Ahmedabad, India.
  • Google Scholar
Cristina Maria Tettamanzi
  • Cristina Maria Tettamanzi
  • Department of Chemistry and Biochemistry, University of the Sciences, Philadelphia, USA.
  • Google Scholar
Jesse Rajan
  • Jesse Rajan
  • Department of Pharmacognosy, L.M. College of Pharmacy, Ahmedabad, India.
  • Google Scholar
Mamta Shah
  • Mamta Shah
  • Department of Pharmacognosy, L.M. College of Pharmacy, Ahmedabad, India.
  • Google Scholar
Bela Peethambaran
  • Bela Peethambaran
  • Department of Chemistry and Biochemistry, University of the Sciences, Philadelphia, USA.
  • Google Scholar


  •  Received: 24 May 2016
  •  Accepted: 19 July 2016
  •  Published: 10 August 2016

Abstract

Achillea millefolium is a flowering plant in the family, Asteraceae and is known to be used in traditional medicine as astringent, stimulant, tonic, antiphlogistic and for the treatment of cold and influenza. In an earlier study in the authors’ laboratory, four different extracts of a variety of yarrow called Achillea ‘Moonshine’ were tested for anti-acne effect. The petroleum ether extract of Achillea ‘Moonshine’ was demonstrated earlier in the authors’ laboratory to have phytochemicals that could scavenge free radicals, inhibit growth of Propionibacterium acnes and reduce tyrosinase activity which are needed for acne treatment. Using column chromatography, the petroleum ether extract was fractionated and each fraction was tested against P. acnes using TLC bioautography. The active compound from TLC bioautography was isolated by HPLC and identified using Nuclear Magnetic Resonance and Mass Spectrometry studies. This led to characterization of a novel piperidide, N-(21-hydroxy-21-(piperidin-1-yl) henicosa-17, 19-diyl-1-yl) acetamide, from the petroleum ether extract of Achillea ‘Moonshine’ that contributed to the anti-acne activity. This study led to identification of a novel natural anti-acne compound that is anti-oxidant, anti-inflammatory and anti-microbial against acne causing organism.

Key words: Achillea ‘Moonshine’, petroleum ether extract, anti-acne activity, piperidides