Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3830

Full Length Research Paper

Refining the methods of extraction and purification of apigenin from Viola yedoensis makino

Ai-wen Dong
  • Ai-wen Dong
  • Key Laboratory of Hunan Forest Products and Chemical Industry Engineering and Jishou University, Zhangjiajie Municipal, Hunan Province, 427000, China.
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Qi Zhuo*
  • Qi Zhuo*
  • College of Rural-Urban Resources and Planning Science, Jishou University, Zhangjiajie Municipal, Hunan Province, 427000, China.
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Xiao-ying Bu
  • Xiao-ying Bu
  • Key Laboratory of Hunan Forest Products and Chemical Industry Engineering and Jishou University, Zhangjiajie Municipal, Hunan Province, 427000, China.
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Shen-hai Zhu
  • Shen-hai Zhu
  • College of Rural-Urban Resources and Planning Science, Jishou University, Zhangjiajie Municipal, Hunan Province, 427000, China.
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Guo-qing Wang
  • Guo-qing Wang
  • Key Laboratory of Hunan Forest Products and Chemical Industry Engineering and Jishou University, Zhangjiajie Municipal, Hunan Province, 427000, China.
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  •  Received: 24 December 2011
  •  Accepted: 18 May 2015
  •  Published: 25 May 2015

Abstract

In this study, the effects of supercritical CO2 extraction and macroporous adsorbent resin on separation and purification of apigenin were compared. A better separation and purification macroporous adsorbent resin was selected according to static adsorption and desorption results. Silica gel column chromatography and LSA-10 macroporous adsorbent resin performed better in the separation and purification procedure. The purity of apigenin could reach 92.8% after separation and purification. The optimal supercritical CO2 extraction conditions of apigenin from Viola yedoensis makino are solid to liquid ratio: 5:3, extraction pressure: 20 MPa, extraction temperature: 55°C, extraction time: 2 h, separation vessel temperature: 55°C, pressure: 9 Mpa, extraction vessel temperature: 40°C and pressure:5 MPa. Supercritical CO2 extraction had a great effect on the separation and purification of apigenin from V. yedoensis makino; the purity could reach 97.6%. Assaying apigenin by high performance liquid chromatography (HPLC) showed that the sample volume and peak area of the response have a good linear relationship, r = 0.9996, between 1.58 to 15.80. The results indicated that the procedure of separation and purification of apigenin from V. yedoensis by supercritical CO2 extraction, which is better than that of macroporous adsorbent resin, is suitable for the separation and purification of high purity apigenin.

 

Key words: Extraction, purification, macroporous adsorbent resin, supercritical CO2 extraction.