Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3672

Full Length Research Paper

Essential oil of Cymbopogon flexuosus, Vernonia polyanthes and potassium phosphite in control of bean anthracnose

Jhonata Lemos da Silva
  • Jhonata Lemos da Silva
  • Instituto Federal de Educação, Ciência e Tecnologia do Amazonas, Humaitá, AM, Brazil.
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Paulo Estevão de Souza
  • Paulo Estevão de Souza
  • Departamento de Fitopatologia, Universidade Federal de Lavras, Lavras, MG, Brazil.
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Eduardo Alves
  • Eduardo Alves
  • Departamento de Fitopatologia, Universidade Federal de Lavras, Lavras, MG, Brazil.
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Jose Eduardo Brasil Pereira Pinto
  • Jose Eduardo Brasil Pereira Pinto
  • Departamento de Agricultura, Universidade Federal de Lavras, Lavras, MG, Brazil.
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Suzan Kelly Vilela Bertolucci
  • Suzan Kelly Vilela Bertolucci
  • Departamento de Agricultura, Universidade Federal de Lavras, Lavras, MG, Brazil.
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Marcelo Loran Oliveira Freitas*
  • Marcelo Loran Oliveira Freitas*
  • Departamento de Fitopatologia, Universidade Federal de Lavras, Lavras, MG, Brazil.
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Camila Cristina Lage de Andrade
  • Camila Cristina Lage de Andrade
  • Departamento de Fitopatologia, Universidade Federal de Lavras, Lavras, MG, Brazil.
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Mario Lucio Vilela Resende
  • Mario Lucio Vilela Resende
  • Departamento de Fitopatologia, Universidade Federal de Lavras, Lavras, MG, Brazil.
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  •  Received: 19 December 2014
  •  Accepted: 12 February 2015
  •  Published: 25 February 2015

Abstract

This study evaluated essential oils of Cymbopogon flexuosus (EOC), Vernonia polyanthes (EOV), and potassium phosphite (PP) in control of bean anthracnose caused by Colletotrichum lindemuthianum. This study assessed mycelial growth and conidial germination in vitro, scanning electron microscopy (SEM), and enzymes peroxidase (POX) and phenylalanine ammonia-lyase (PAL). EOC and EOV in doses 1,450 and 1,320 μL L-1 reduced disease severity by 57.2 and 37.6%, respectively. Major components identified in EOC were geraniol 46.8% and nerol 33.4%, and germacrene-D (42.2%) and bicyclogermacrene (17.2%) in EOV. SEM images showed that PP, EOC and EOV reduced mycelial growth and emission of germ tubes. PP and EOC increased POX and PAL rates in bean tissue. Bean anthracnose was controlled by direct antifungal activity of PP and EOC and induction of defense enzymes

 

Key words: Alternative disease control, Colletotrichum lindemuthianum, resistance induction, scanning electron microscopy, Phaseolus vulgaris.