Abstract

A protocol for in vitro propagation from leaf explants of Barringtonia racemosa is reported. Calli were aseptically raised by placing surface sterilized leaf explants on Woody Plant Medium (WPM) supplemented with different concentrations of    2, 4-dichlorophenoxyacetic acid (2,4-D). On the shoot induction medium, the callus induced on the WPM medium containing 2 mg/L (w/v) KIN+0.2 mg/l (w/v) IBA and    2 mg/L (w/v) of KIN +0.4 mg/L(w/v) of NAA was the most effective, providing high shoot regeneration frequency of 85.6 and 76.5% respectively. In addition, the highest number of shoots produced was 8.2 and 6.3 shoots per explant respectively in the medium containing the mentioned phytohormones. The good rooting percentage 62 and 5.6% roots per shoot were achieved on WPM medium supplemented with 3g/L (w/v) of active charcoal and 0.8 mg/L (w/v) of IBA. Approximately 96% of the in vitroraised plantlets with well developed shoots and roots were survived after transferring to mixed soil and grown well in the glass house.

Keywords:  Barringtonia racemosa, rooting, shoot regeneration.