Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3835

Full Length Research Paper

Genetic diversity in two Lilium (Liliaceae) species from different regions of Greece based on a Random Amplified Polymorphic DNA (RAPD) analysis

Nursel Ä°kinci
Department of Biology, Faculty of Arts and Sciences, Abant Izzet Baysal University, TR-14280, Bolu, Turkey
Email: [email protected], [email protected]

  •  Accepted: 25 August 2010
  •  Published: 18 September 2010

Abstract

Distribution of genetic variation between populations of Lilium albanicum andLilium chalcedonicum was investigated using RAPD (random amplified polymorphic DNA) profiles. Plants for the analysis were collected from four different populations, containing 33 individuals. Eleven primers yielded 88 polymorphic bands. UPGMA clustering and principle coordinate analysis indicated a clear separation between the populations of the two species and all the analyzed individuals clustered in three groups, one comprising L. chalcedonicum and the other two groups encompassing three populations of L. albanicum. The analysis of molecular variance (AMOVA) indicated that 33% of the total variance is between species. The highest amount of genetic differentiation, 51% of the total variance, is found within the individual populations.

 

Key words: Liliaceae, Lilium albanicumLilium chalcedonicum, RAPD, genetic variation.