Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3582

Full Length Research Paper

In vitro antibacterial and antidiarraheic activity of root bark extract of Anogeissus leiocarpa (Combretaceae) during an experimental bacterial diarrhea induced by Escherichia coli extended-spectrum β-lactamases (ESBL) in albino Wistar rats

Kouangbe Mani Adrien
  • Kouangbe Mani Adrien
  • Biochemical Pharmacodynamy Laboratory, Training and Research Unit of Biosciences, University Félix Houphouët-Boigny, P. O. Box 582 Abidjan 22, Abidjan, Côte d’Ivoire.
  • Google Scholar
Sonan Kouadio Henri Guillaume
  • Sonan Kouadio Henri Guillaume
  • Department of Biochemistry-Genetics, Training and Research Unit of Biological Sciences, University Peleforo Gon Coulibaly, P. O. Box 1328 Korhogo, Korhogo, Côte d’Ivoire,
  • Google Scholar
Meite Souleymane
  • Meite Souleymane
  • Department of Clinical and Basic Biochemistry, Pasteur Institute of Côte d’Ivoire, P. O. Box 490 Abidjan 01, Abidjan, Côte d’Ivoire.
  • Google Scholar
Boga Gogo Lucien
  • Boga Gogo Lucien
  • Biochemical Pharmacodynamy Laboratory, Training and Research Unit of Biosciences, University Félix Houphouët-Boigny, P. O. Box 582 Abidjan 22, Abidjan, Côte d’Ivoire.
  • Google Scholar
N’Guessan Jean David
  • N’Guessan Jean David
  • Biochemical Pharmacodynamy Laboratory, Training and Research Unit of Biosciences, University Félix Houphouët-Boigny, P. O. Box 582 Abidjan 22, Abidjan, Côte d’Ivoire.
  • Google Scholar


  •  Received: 18 August 2018
  •  Accepted: 05 September 2018
  •  Published: 10 November 2018

Abstract

The present study was conducted to investigate in vitro and in vivo antibacterial activity of the root bark extracts of Anogeissus leiocarpa (DC) Guill. & Perr, in Escherichia coli extended-spectrum β-lactamases (ESBL)-induced diarrhea in rat. The antibacterial activity was performed in vitro by determining the inhibition zone using standard agar diffusion method as well as in vivo on E. coli infected Wistar rat model. Both minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were carried through microdilution method. Results obtained in this study indicated that ethanolic and acetatic extracts were only active on bacteria presenting an inhibition zone range from 8 to 16 mm. The MIC observed in agar slant tubes ranged from 6.25 to 50 mg/ml. The ethanolic fraction of A. leiocarpa (ETHA) showed the highest in vitro antibacterial activity against strains with MICs ranging from 6.25 to 12.5 mg/ml and MBCs ranging from 12.5 to 25 mg/ml. In vivo, after infection, diarrhea increased faeces frequency, weight and volume faeces and bacterial faeces load to a maximum on the 2nd day after infection (P < 0.01). ETHA normalized the appearance, weight, volume and water content of faeces. To all doses, like ciprofloxacin it reduced significantly (P < 0.05) the bacterial growth compared to control Lot (infected and untreated). The death rate in diarrheic control Lot was 50% by Day 14. No death was recorded in Lot treated with ciprofloxacin and Lot treated with ethanolic fraction at dose of 2222.22 mg/kg body weight (bw). This study supports the use of A. leiocarpa in the traditional treatment of bacterial infections and offer many perspectives in the search for new molecules against resistant microbial strains.

Key words: Anogeissus leiocapa, antibacterial activity, in vitro, in vivo, therapeutic.

 

Abbreviation

ETHA, Ethanol fraction of Anogeissus leiocarpa; ETAA, total aqueous extract of Anogeissus leiocarpa; EDMA, dichloromethane fraction of Anogeissus leiocarpa; EAA, ethyl acetate fraction of Anogeissus leiocarpa.