Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3835

Full Length Research Paper

Effect of hexane extract of Dillenia indica seed on the activity of secreted aspartyl proteinase of Candida albicans and its kinetic studies

K. Himakar Reddy1*, Patrick Govender1, O. V. S. Reddy2 and P.V. G. K. Sarma3
1Department of Biochemistry, University of KwaZulu-Natal, Durban-4000, South Africa 2Department of Biochemistry, Sri Venkateswara University, Tirupati-517 502, India. 3Department of Biotechnology, Sri Venkateswara Institute of Medical Sciences, Tirupati, India
Email: [email protected]

  •  Accepted: 31 January 2011
  •  Published: 17 November 2012

Abstract

Secreted aspartyl proteinase (Sap), an extracellular enzyme of Candida albicans, is implicated as a pathogenic factor that plays a vital role in invading and colonizing the host tissue under predisposing conditions. The present study was taken up to study the inhibitory effect of hexane extract of Dillenia indica seed on Sap and its enzyme kinetics. Sap activity was determined spectrophotometrically at 280 nm using BSA. The enzyme kinetics of Sap showed velocity (Vmax) of 0.058 µg/mg/min and Km 0.174 µM. However C. albicans grown in different concentrations of hexane extract of D. indica totally inactivated the enzyme, which was further confirmed by SDS-PAGE.

 

Key words: Dillenia indica, Candida albicans, secreted aspartyl proteinase, Michaelis-menten, Hanes-Woolf.

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