Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3789

Full Length Research Paper

Anti-HIV-1 reverse transcriptase activities of hexane extracts from some Asian medicinal plants

Kun Silprasit1, Supaporn Seetaha2, Parinya Pongsanarakul2, Supa Hannongbua3 and Kiattawee Choowongkomon2,4*
1Faculty of Environmental culture and Ecotourism, Srinakharinwirot University, Wattana, Bangkok 10110, Thailand. 2Department of Biochemistry, Faculty of Science, Kasetsart University, Bangkean, Bangkok 10900, Thailand. 3Department of Chemistry, Faculty of Science, Kasetsart University, Bangkean, Bangkok 10900, Thailand. 4Center for Advanced Studies in Tropical Natural Resources, National Research University-Kasetsart University, Kasetsart University, Bangkok, 10900, Thailand, CASTNAR, NRU-KU, Thailand.
Email: [email protected]

  •  Accepted: 05 July 2011
  •  Published: 23 September 2011

Abstract

Numerous herbal remedies against viral infections, including HIV-1, have been used as a part of Asian traditional medicine. However, the viral protein targets for inhibition by these medications are less well identified. HIV-1 reverse transcriptase is one of the targets, since high-efficiency inhibitors such as non-nucleoside HIV-1 reverse transcriptase inhibitor have been successfully used. In this work, hexane extracts of Asian medicinal plants were screened against HIV-1 reverse transcriptase. The results showed that the crude extracts from plants Cinnamomum loureiroi (stem bark), Quercus infectoria (fruit), Plumbago indica L.(root), Artocarpus heterophyllus Lam. (seed), Ocimum sanctum L. (leaves), Allium sativum L.(bulb) and Acorus calamus L. (rhizomes) showed strong HIV-1 reverse transcriptase inhibitory effects. The efficiency of anti-HIV-1RT activity was reported as 50% inhibitory concentrations (IC50). This showed that the hexane crude extracts from A. calamus L. andA. heterophyllus Lam. contained potent activity against HIV-1 RT, with IC50 of 32.96 ± 3.17 and 34.69 ± 2.41 µg/ml, respectively.

 

Key words:  Anti-HIV agents, HIV-1 reverse transcriptase, inhibitor screening, PicoGreen.