Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3834

Full Length Research Paper

Protective effect of extracts of calyx from Diospyros kaki on H2O2-induced cytotoxicity in PC12 cells

Mi-Young Yoon1,3, Eun-Ah Lee1, Ae-Ran Choi1, Seong-Hee Moon1, Yongseong Kim2and Hae-Ryong Park1*    
1Department of Food Science and Biotechnology, Kyungnam University, Changwon 631-701, Korea. 2Department of Science Education, Kyungnam University, Changwon 631-701, Korea. 3Eco-friendly New Materials Research Group, Korea Research Institute of Chemical Technology, Daejeon 305-605, Korea.      
Email: [email protected]

  •  Accepted: 10 December 2012
  •  Published: 10 March 2013

Abstract

Oxidative stress is considered a major cause of cellular injuries, including various clinical abnormalities. Here, the neuroprotective effect of extracts from the calyx of Diospyros kaki(DCE), peel of D. kaki (DPE), and flesh of D. kaki (DFE) of Diospyros kaki on oxidative stress-induced apoptosis in PC12 cells was investigated by an 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) reduction assay. The results showed that H2O2significantly decreased cell viability (50.9%), and DCE exerted the highest neuroprotective effect on H2O2-induced cytotoxicity. We identified that treatment with DCE of H2O2-stressed PC12 cells caused dose-dependent suppression of H2O2-induced leakage of lactate dehydrogenase (LDH) as released amount (11.1 to 30.4%); we further verified these findings by observing morphological features. H2Oalso induced severe apoptosis of the PC12 cells, as determined by Hoechst 33342 staining and flow cytometric analysis. DCE (100, 500 µg/ml) exerted a significantly high protective effect on PC12 cells against H2O2-induced cell injury, and the percentage of cells in the sub-G1 phase decreased to 3.7 and 4.6%. These results suggest that DCE can protect cells against H2O2-induced apoptosis, and might be a potential therapeutic agent for treating or preventing neurodegenerative diseases that are related to oxidative stress.

 

Key words: Apoptosis, Diospyros kaki, neurodegenerative disease, neuroprotective effect, oxidative stress.