Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3833

Full Length Research Paper

Relationship between mycelium morphology and extracellular polysaccharide production of medicinal mushroom Ganoderma lucidum in submerged culture

Zhongyang Ding1,3*, Qiong Wang1,3, Lin Peng2,3, Liang Zhang2,3, Zhenghua Gu2,3, Guiyang Shi2,3 and Kechang Zhang2
1The Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China. 2The Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China. 3National Engineering Laboratory for Cereal fermentation Technology, Jiangnan University, Wuxi 214122, China.
Email: [email protected]

  •  Accepted: 22 November 2011
  •  Published: 16 April 2012

Abstract

The medicinal mushroom Ganoderma lucidum is a source of polysaccharides used in disease treatment. The objective of this study was to investigate the relationships betweenG. lucidum mycelium morphology and the production of extracellular polysaccharides (EPS) in submerged fermentation. Mycelium pellets were classified according to diameter as either S, M or L pellets. M pellets were the main source of EPS. Changes in mycelium morphology and EPS production were obtained when cells were grown in different culture media; medium that contained a high concentration of glucose and low peptone enhanced the formation of M pellets. The proportion of M pellets in the culture was influenced greatly by KH2PO4 concentration and increase was accompanied by enhanced EPS production. Dry cell weight was affected mostly by the percentage of total M and L pellets present, while S pellet numbers had little effect. The production of EPS and the accumulation of mycelia were influenced mainly by G. lucidum pellet morphology. This study suggests that in large scale fermentation, high yields of EPS would be obtained by the control of mushroom morphology.

 

Key words: Medicinal mushroom, Ganoderma lucidum, morphology, extracellular polysaccharides, submerged fermentation.