Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3828

Full Length Research Paper

Comparison of in vitro antimicrobial effect of ethanol extracts of Satureja khuzestanica, Rhus coriaria, and Ocimum basilicum L. on Helicobacter pylori

Yousef Motaharinia1, Mohammad Saleh Hazhir2, Mohammad Ali Rezaee1, Siamak Vahedi3, Ahmad Rashidi1, Werya Hosseini1, Mohammad Saeed Hakhamaneshi2 and Mohammad Reza Rahmani1*
1Department of Immunology, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran. 2Department of Biochemistry and Nutrition, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran. 3Department of Internal Medicine, Faculty of medicine, Besat Hospital, Kurdistan University of Medical Sciences, Sanandaj, Iran.
Email: [email protected]

  •  Accepted: 17 February 2012
  •  Published: 09 June 2012


Helicobacter pylori is the main reason of gastritis, gastric ulcer and cancer, which cause many therapeutic problems for man, throughout the world. Therefore, the aim of the current study is to compare the antimicrobial effect of the extracts of Satureja khuzestanicaRhus coriaria, and Ocimum basilicum Lon H. pylori. To isolate H. pylori, sampling was performed in 30 patients of the endoscopy ward of Be’sat Hospital, Sanandaj. Ethanol extracts of S. khuzestanicaR. coriaria and O. basilicum Lwere prepared. Using disc diffusion and agar dilution methods, the antimicrobial effects of the plant extracts against H. pylori were evaluated. Seven strains of H. pylori were isolated from the biopsy samples. The results of disc diffusion demonstrated that the mean diameter of no-growth halo for the discs containing 20 mg of S. khuzestanicaR. coriaria and O. basilicum L. were ≥ 12.28, ≥ 19.42, and ≥ 11.42 mm, respectively. The values for the discs containing 40 mg of the extracts were ≥ 20.14, ≥ 28.57, and ≥ 13.57 mm, respectively. The mean diameter of no-growth halo for gentamicin (10 μg/ml con.) as the positive control was obtained to be ≥ 37.85 mm. The results of agar dilution method showed that the minimum inhibitory concentration (MIC) of the extracts of S. khuzestanicaR. coriaria, and O. basilicum L. were 307.14, 214.28, and 392.8 µg/ml, respectively. The study showed that the ethanol extract ofR. coriaria and S. khuzestanica had antibacterial effect against H. pylori.


Key words: Satureja khuzestanica, Rhus coriaria, Ocimum basilicum L., H. pylori,antibacterial effect.