Abstract

Helicobacter pylori is the main reason of gastritis, gastric ulcer and cancer, which cause many therapeutic problems for man, throughout the world. Therefore, the aim of the current study is to compare the antimicrobial effect of the extracts of Satureja khuzestanica, Rhus coriaria, and Ocimum basilicum L. on H. pylori. To isolate H. pylori, sampling was performed in 30 patients of the endoscopy ward of Be’sat Hospital, Sanandaj. Ethanol extracts of S. khuzestanica, R. coriaria and O. basilicum L. were prepared. Using disc diffusion and agar dilution methods, the antimicrobial effects of the plant extracts against H. pylori were evaluated. Seven strains of H. pylori were isolated from the biopsy samples. The results of disc diffusion demonstrated that the mean diameter of no-growth halo for the discs containing 20 mg of S. khuzestanica, R. coriaria and O. basilicum L. were ≥ 12.28, ≥ 19.42, and ≥ 11.42 mm, respectively. The values for the discs containing 40 mg of the extracts were ≥ 20.14, ≥ 28.57, and ≥ 13.57 mm, respectively. The mean diameter of no-growth halo for gentamicin (10 μg/ml con.) as the positive control was obtained to be ≥ 37.85 mm. The results of agar dilution method showed that the minimum inhibitory concentration (MIC) of the extracts of S. khuzestanica, R. coriaria, and O. basilicum L. were 307.14, 214.28, and 392.8 µg/ml, respectively. The study showed that the ethanol extract ofR. coriaria and S. khuzestanica had antibacterial effect against H. pylori.

Key words: Satureja khuzestanica, Rhus coriaria, Ocimum basilicum L., H. pylori,antibacterial effect.