Attempts were made to evaluate suitable cultural conditions for micropropagation ofDalbergia sissoo from nodal meristem. The best shoot formation response (88%) was obtained on Murashige and Skoog (MS) medium (Murashige and Skoog, 1962) containing 1.0 mg/l 6-benzylamino-purine (BAP) + 0.25 mg/l α- naphthalene acetic acid (NAA) after 10.8 days of explants inoculation and average shoot length was recorded to be 2.4 cm after 25 days . For in vitro shoot multiplication, MS medium inferred with 1.5 mg/l BAP + 0.25 mg/l Kinetin exhibited maximum number of shoot, that is, 4.0 shoots per culture vial were formed with an average shoot length of 1.78 cm after 4 weeks of inoculation. For rooting of well developed in vitro shoots, optimum results were obtained on MS medium supplemented with 1.0 mg/l of indole butyric acid (IBA) which were 3.4 roots per plant with an average root length of 1.8 cm after 24 days of inoculation. During all this investigation, different temperature conditions ranging from 23 to 30°C were provided to the cultures, however, optimum results were obtained at 26 ± 1°C at 16/8 h (light/dark) photoperiod.
Key words: Dalbergia sissoo, tissue culture, micro propagation, culture media, shoot multiplication, in vitro rooting.
MS, Murashige and Skoog’s medium; BAP, 6-benzylamino-purine; Kin,kinetin; IAA, indole acetic acid NAA, α- naphthalene acetic acid, IBA, indole butyric acid;HCl, hydrochloric acid; NaOH, sodium hydroxide.
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