Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3743

Full Length Research Paper

Extracts and gel formulations of Spondias mombin L. stem and its main compound ellagic acid reduce cutaneous inflammation in experimental models

Karla Michelli Garcia de Oliveira
  • Karla Michelli Garcia de Oliveira
  • Department of Pharmaceutical Sciences, Faculty of Pharmacy, Federal University of Juiz de Fora, Campus Universitário, São Pedro, Juiz de Fora, Minas Gerais, 36036-900, Brazil.
  • Google Scholar
Fernanda Kelly Costa
  • Fernanda Kelly Costa
  • Department of Pharmaceutical Sciences, Faculty of Pharmacy, Federal University of Juiz de Fora, Campus Universitário, São Pedro, Juiz de Fora, Minas Gerais, 36036-900, Brazil.
  • Google Scholar
Ana Lúcia Santos de Matos Araújo
  • Ana Lúcia Santos de Matos Araújo
  • Department of Pharmaceutical Sciences, Faculty of Pharmacy, Federal University of Juiz de Fora, Campus Universitário, São Pedro, Juiz de Fora, Minas Gerais, 36036-900, Brazil.
  • Google Scholar
Fernanda Maria Pinto Vilela
  • Fernanda Maria Pinto Vilela
  • Department of Pharmaceutical Sciences, Faculty of Pharmacy, Federal University of Juiz de Fora, Campus Universitário, São Pedro, Juiz de Fora, Minas Gerais, 36036-900, Brazil.
  • Google Scholar
Mirian Pereira Rodarte
  • Mirian Pereira Rodarte
  • Department of Pharmaceutical Sciences, Faculty of Pharmacy, Federal University of Juiz de Fora, Campus Universitário, São Pedro, Juiz de Fora, Minas Gerais, 36036-900, Brazil.
  • Google Scholar
Maria Silvana Alves
  • Maria Silvana Alves
  • Department of Pharmaceutical Sciences, Faculty of Pharmacy, Federal University of Juiz de Fora, Campus Universitário, São Pedro, Juiz de Fora, Minas Gerais, 36036-900, Brazil.
  • Google Scholar
Célia Hitomi Yamamoto
  • Célia Hitomi Yamamoto
  • Department of Pharmaceutical Sciences, Faculty of Pharmacy, Federal University of Juiz de Fora, Campus Universitário, São Pedro, Juiz de Fora, Minas Gerais, 36036-900, Brazil.
  • Google Scholar
Orlando Vieira de Sousa
  • Orlando Vieira de Sousa
  • Department of Pharmaceutical Sciences, Faculty of Pharmacy, Federal University of Juiz de Fora, Campus Universitário, São Pedro, Juiz de Fora, Minas Gerais, 36036-900, Brazil.
  • Google Scholar


  •  Received: 16 February 2020
  •  Accepted: 18 March 2020
  •  Published: 30 April 2020

Abstract

Spondias mombin L. is a medicinal plant traditionally used for the treatment of vomiting, diarrhea, hemorrhoids, wounds and inflammation. In this study, the topical anti-inflammatory activity of S. mombin stem and ellagic acid was investigated. From the dried stem, powdered bark and wood were separately subjected to ethanol extraction by static maceration to obtain the EESB (bark) and EESW (wood) extracts. These extracts were analyzed by TLC and HPLC-DAD-UV. Using EESB and EESW (0.1, 0.5 and 1 mg/ear), GEESB and GEESW gel formulations (1, 3 and 5%) and ellagic acid (EA - 1 mg/ear), the topical anti-inflammatory activity was carried out by Croton oil-induced ear edema. Histopathological analysis, myeloperoxidase (MPO), N-acetyl-β-D-glucosaminidase (NAG) and nitric oxide (NO) were assessed. EA was identified in EESB and EESW as the main compound. After 6 h of treatment, EESB (53.83 to 63.06%), GEESB (41.45 to 43.48%), EESW (37.51 to 49.30%), GEESW (45.02 to 50.70%), and EA (58.59%) inhibited the ear thickness. In 24 h, the ear thickness was reduced by EESB (56.22 to 58.01%), GEESB (44.79 to 60.67%), EESW (44.11 to 62.69%), GEESW (49.38 to 62.88%) and EA (62.90%). In this time, ear weight, MPO, NAG, NO, leukocyte migration and vasodilation were decreased. These results suggest that S. mombin stem has topical anti-inflammatory activity, which supports the traditional uses and may represent an alternative for the treatment of cutaneous inflammation.

Key words: Spondias mombin, ellagic acid, topical formulation, inflammation, anti-inflammatory agents.