Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3755

Full Length Research Paper

Phyllanthin and hypophyllanthin determination by gas chromatography-mass spectrometry of six stonebreaker species from different regions of Brazil

Kátia Viviane Alves Novellino
  • Kátia Viviane Alves Novellino
  • Departamento de Produtos Naturais, Instituto de Tecnologia em Fármacos, Farmanguinhos, Fundação Oswaldo Cruz. Av. Brasil, 4365, Manguinhos, Rio de Janeiro, RJ - 21040-900, Brasil.
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Suellen Rodrigues Bernardo
  • Suellen Rodrigues Bernardo
  • Departamento de Métodos Analíticos, Instituto de Tecnologia em Fármacos, Farmanguinhos, Fundação Oswaldo Cruz. Av. Brasil, 4365, Manguinhos, Rio de Janeiro, RJ - 21040-900, Brasil.
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Marcelo Raul Romero Tappin
  • Marcelo Raul Romero Tappin
  • Departamento de Métodos Analíticos, Instituto de Tecnologia em Fármacos, Farmanguinhos, Fundação Oswaldo Cruz. Av. Brasil, 4365, Manguinhos, Rio de Janeiro, RJ - 21040-900, Brasil.
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Rosa de Belém das Neves Alves
  • Rosa de Belém das Neves Alves
  • Recursos Genéticos e Biotecnologia, Empresa Brasileira de Pesquisa Agropecuária, Embrapa, Parque Estação Biológica - PqEB s/n°, Brasília, DF - 70770-901, Brasil.
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Dijalma Barbosa da Silva
  • Dijalma Barbosa da Silva
  • Recursos Genéticos e Biotecnologia, Empresa Brasileira de Pesquisa Agropecuária, Embrapa, Parque Estação Biológica - PqEB s/n°, Brasília, DF - 70770-901, Brasil.
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Marcos José da Silva
  • Marcos José da Silva
  • Departamento de Botânica,Instituto de Ciências Biológicas I, Universidade Federal de Goiás, UFG, Campus Samambaia II, Sala 204. Setor Itatiaia, Goiânia, GO - 74001-970, Brasil.
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Roberto Fontes Vieira
  • Roberto Fontes Vieira
  • Recursos Genéticos e Biotecnologia, Empresa Brasileira de Pesquisa Agropecuária, Embrapa, Parque Estação Biológica - PqEB s/n°, Brasília, DF - 70770-901, Brasil.
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Maria das Dores Dutra Behrens
  • Maria das Dores Dutra Behrens
  • Departamento de Produtos Naturais, Instituto de Tecnologia em Fármacos, Farmanguinhos, Fundação Oswaldo Cruz. Av. Brasil, 4365, Manguinhos, Rio de Janeiro, RJ - 21040-900, Brasil.
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Davyson de Lima Moreira
  • Davyson de Lima Moreira
  • Departamento de Produtos Naturais, Instituto de Tecnologia em Fármacos, Farmanguinhos, Fundação Oswaldo Cruz. Av. Brasil, 4365, Manguinhos, Rio de Janeiro, RJ - 21040-900, Brasil.
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  •  Received: 17 January 2020
  •  Accepted: 13 March 2020
  •  Published: 30 April 2020

Abstract

Stonebreaker is a medicinal plant traditionally used for the treatment of urolithiasis among other illnesses. However, several species of the genus Phyllanthus L. are called “quebra-pedra” (stonebreaker) by the general population in Brazil, due to taxonomic similarities, although the use of a species with a low content of active compounds may lead to a less effective treatment. P. niruri and P. tenellus are recognized as stonebreaker in the Brazilian Pharmacopoeia, and the Brazilian Ministry of Health included Phyllanthus spp in a list of medicinal plants recommended for research and development, indicating the need for further studies. Quantitative determination of the active compounds of different Phyllanthus species from Brazil is important because it allows to assess the impact of their role in public health and so contribute to the goal of RENISUS, the official list of species of interest to the national health service. Lignans comprise a group of compounds found in Phyllanthus species that have diverse pharmacological properties. Some of the most studied lignans are phyllanthin and hypophyllanthin, which have antihyperuricemic activity. The aim of this study was to develop an analytical method for the quantitative determination of phyllanthin and hypophyllanthin in Phyllanthus species by GC-MS and to assess the content of these lignans in six species of the genus from four different regions of Brazil. The phyllanthin and hypophyllanthin contents were found to be very variable in the analyzed species, ranging from not detectable or a mere trace to 0.6% (m/m), the highest value, found in P. amarus.

Key words: Traditional medicine, urolithiasis, stonebreaker, quebra-pedra, Phyllanthus, lignans, gas chromatography-mass spectrometry (GC-MS)