Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3835

Full Length Research Paper

Anti-proliferative activities of Centella asiatica extracts on human respiratory epithelial cells in vitro

Mohd Heikal, M. Y.
  • Mohd Heikal, M. Y.
  • Tissue Engineering Centre, UKM Medical Centre, Jalan Yaacob Latiff, Bandar Tun Razak, 56000 Cheras, Kuala Lumpur, Malaysia., Department of Physiology, Medical Faculty UKM, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia.
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Siti Mariam, H
  • Siti Mariam, H
  • Tissue Engineering Centre, UKM Medical Centre, Jalan Yaacob Latiff, Bandar Tun Razak, 56000 Cheras, Kuala Lumpur, Malaysia.
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Mohd Ilham, A.
  • Mohd Ilham, A.
  • Malaysian Institute of Pharmaceuticals and Nutraceuticals (IPHARM) Persiaran Bukit Jambul, 11900 Bayan Lepas, Pulau Pinang, Malaysia., Forest Research Institute of Malaysia (FRIM), Kepong, 52109 Selangor, Malaysia.
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Mee Fong, C.
  • Mee Fong, C.
  • Tissue Engineering Centre, UKM Medical Centre, Jalan Yaacob Latiff, Bandar Tun Razak, 56000 Cheras, Kuala Lumpur, Malaysia.
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Aminuddin, B. S.
  • Aminuddin, B. S.
  • Tissue Engineering Centre, UKM Medical Centre, Jalan Yaacob Latiff, Bandar Tun Razak, 56000 Cheras, Kuala Lumpur, Malaysia., Ear, Nose & Throat Consultant Clinic, Ampang Puteri Specialist Hospital, Taman Dato Ahmad Razali, 68000 Ampang, Selangor, Malaysia.
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Ruszymah BHI
  • Ruszymah BHI
  • Tissue Engineering Centre, UKM Medical Centre, Jalan Yaacob Latiff, Bandar Tun Razak, 56000 Cheras, Kuala Lumpur, Malaysia., Department of Physiology, Medical Faculty UKM, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia.
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  •  Received: 17 April 2012
  •  Accepted: 19 June 2014
  •  Published: 25 June 2014

Abstract

Centella asiatica or “pegaga” is well known for its ability in promoting wound healing. This study focused on the effect of C. asiatica on the proliferation of human respiratory epithelial (RE) cells. RE cells were cultured using co-culture techniques until first passage (P1). Viability cell test by tryphan blue dye exclusion assay showed that there was high percentage of cell viability at both P0 (74%) and P1 (91.61%). Triplicate tetrazolium dye (MTT assays) were carried out with different concentrations of C. asiatica from 15.6, 31.3, 62.5, 125, 250, 500, 1000, until 2000 ppm. The higher the concentration of C. asiatica, the more inhibitory effect was seen. C. asiatica aqueous extract at concentration 1000 and 2000 ppm demonstrated a significant (p < 0.05) inhibitory effect on human RE cells proliferation on day 4 and 7 after treatment. This provides potential use of C. asiatica extract for the treatment of conditions with respiratory epithelial cells overgrowth.

 

Key words: Centella asiatica, respiratory epithelial cells, anti-proliferative.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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