Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3833

Full Length Research Paper

In vitro and in vivo investigations of the wound healing effect of crude Spirulina extract and C-phycocyanin

Canan Sevimli Gur1,5, Deniz Kiraz Erdogan2, Ilyas Onbasılar3, Pergin Atilla4, Nur Cakar4 and Ismet Deliloglu Gurhan1*    
1Department of Bioengineering, Faculty of Engineering, Ege University, Bornova, 35100 Izmir, Turkey. 2Quintiles Diagnostic Researches, 34337 Istanbul, Turkey. 3Experimental Animal Research and Husbandry Unit, Faculty of Medicine, Hacettepe University, 06100 Ankara, Turkey. 4Department of Histology and Embryology, Faculty of Medicine, Hacettepe University, 06100 Ankara, Turkey. 5Kocaeli University, Science and Literature Faculty, Department of Biology, Biotechnology Discipline, Kocaeli, Turkey.    
Email: [email protected].

  •  Accepted: 17 October 2012
  •  Published: 25 February 2013

Abstract

The aim of this study was to evaluate the influence of crude Spirulina extract and C-phycocyanin (C-PC) isolated from crude Spirulina extract on cultured human keratinocyte, using in vitro and in vivo models of wound healing. Spirulina has been used as a nutraceutical and source of potential pharmaceuticals, however it is not known which component of the cyanobacteria is effective for wound healing. In in vitro model, cultured human keratinocyte were used to investigate the effects of crude Spirulina extract (PSE) and C-phycocyanin (C-PC) extracts on processes involved in keratinocyte proliferation, regeneration and migration. Keratinocyte proliferation and regeneration were monitored by the colorimetric (MTT) assay and migration was monitored in relation to the closure of a denuded area scratched in a confluent monolayer. On the other hand, in in vivo model using Sprague-Dawley male rats, effects of PSE and C-PC on tissue regeneration were investigated. Results of in vivo wound healing study were monitored by means of histological examinations. PSE extract showed the best growth stimulation at 33.5 µg/mL dose of treatment, which revealed a cell viability ranging from 100 to 270% after 72 h. Cell viability was also good for C-PC and was measured as high as 213%. Cell viability and proliferation difference between PSE and C-PC were observed not to be significant (p > 0.05) at the range of doses (33.5 to 0.0335 µg/mL) studied. In in vivo efficiency of the PSE and C–PC, it was observed that 1.25% C-PC has a better effect on the 7th day compared to other preparations.

 

Key words: Spirulina extractc-phycocyanin, in vitroin vivo, Sprague-Dawley rats, 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), tissue regeneration.