Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3693

Full Length Research Paper

Genetic diversity and seasonal chemical profile by 1H NMR and cytotoxic activity in Opuntia and Nopalea genres

Francisco Abel Lemos Alves
  • Francisco Abel Lemos Alves
  • Agronomic Institute of Pernambuco, Av. General San Martin, 1371, Bongi, 50761-000, Recife-PE, Brazil.
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Albericio Pereira de Andrade
  • Albericio Pereira de Andrade
  • Federal University of Paraíba, Centre of Agricultural Sciences, Campus II, 58397-000, Areia-PB, Brazil.
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Riselane de Lucena Alcantara Bruno
  • Riselane de Lucena Alcantara Bruno
  • Federal University of Paraíba, Centre of Agricultural Sciences, Campus II, 58397-000, Areia-PB, Brazil.
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Maria Goretti de Vasconcelos Silva
  • Maria Goretti de Vasconcelos Silva
  • Federal University of Ceará, Laboratory of Natural Products and Medicinal Chemistry, Science Center, 60455-970, Fortaleza-CE, Brazil.
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Maria de Fatima Vanderlei de Souza
  • Maria de Fatima Vanderlei de Souza
  • Federal University of Paraíba, Laboratory of Pharmaceutical Technology, Health Sciences Centre, 58051-970, João Pessoa-PB, Brazil.
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Claudia Pessoa
  • Claudia Pessoa
  • Federal University of Ceará, Health Sciences Center, Department of Physiology and Pharmacology, 60431-970, Fortaleza-CE, Brazil.
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Fatima de Cassia Evangelista de Oliveira
  • Fatima de Cassia Evangelista de Oliveira
  • Federal University of Ceará, Health Sciences Center, Department of Physiology and Pharmacology, 60431-970, Fortaleza-CE, Brazil.
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Severino Goncalves de Brito Filho
  • Severino Goncalves de Brito Filho
  • Federal University of Paraíba, Laboratory of Pharmaceutical Technology, Health Sciences Centre, 58051-970, João Pessoa-PB, Brazil.
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Djalma Cordeiro dos Santos
  • Djalma Cordeiro dos Santos
  • Agronomic Institute of Pernambuco, Av. General San Martin, 1371, Bongi, 50761-000, Recife-PE, Brazil.
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  •  Received: 22 July 2016
  •  Accepted: 12 October 2016
  •  Published: 25 October 2016

Abstract

The cactus pear (Opuntia spp.) is known to have bioactive compounds which work in the prevention of various diseases, especially cancer. The objectives of the study were to characterize the chemical profile and genetic diversity, through chromatic tests and 1H NMR, using multivariate analysis, and assess the cytotoxic potential of cactus pear varieties of Opuntia and Nopalea genera grown in the semi-arid region of Brazil in dry and wet seasons. In the study of chemical prospecting and cytotoxic activity, crude ethanol extracts from cladodes of varieties (IPA-100003, IPA-100004, IPA-200021, IPA-200205, IPA-200008, IPA-200149 and IPA-200016) were used. The cytotoxic activity was evaluated by the MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl bromide tetrazolin] against HCT-116 (human colorectal), SF-295 (human glioblastoma) and OVCAR-8 (human ovarian cancer). The group of chemicals which stand out are the carbohydrates and glycosylated substances, lipids (fatty acids and steroids) and phenolic compounds (flavonoids), which vary both between the botanical varieties studied and among the collection periods. The analysis of the NMR spectra of 1H cactus pear varieties by methods of multivariate analysis finds genetic diversity among the materials in the dry and rainy seasons. The ethanol extract (50 μg ml-1) shows limited growth inhibitory effect on the cancer cell lines examined. It is concluded that the cytotoxic activity of cactus pear cladodes is attributed to phenolic compounds especially the flavonoids. It is recommended that population at risk utilize these materials as preventative natural dietary supplements against cancer.

 

Key words: Anticancer, cactus pear, flavonoids, food analysis, forage, phenolics, semiarid.