Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3834

Full Length Research Paper

Antiangiogenic activity of extracts and fractions from an endemic plant Ardisia pyramidalis (Cav.) Pers. From Bataan, Philippines using Duck in ovo chorioallantoic membrane assay

Annabelle A. Herrera1 and Evangeline C. Amor2
1Institute of Biology, College of Science, UP Diliman, Philppines. 2Institute of Chemistry, College of Science, UP Diliman, Philppines.
Email: [email protected]

  •  Accepted: 06 October 2010
  •  Published: 04 July 2011


Using bioassay directed fractionation, the angiosuppressive activity of leaf extracts ofArdisia pyramidalis (Myrsinaceae) was determined using the duck in ovo chorioallantoic membrane (CAM) assay. The methanol extract was antiangiogenic as indicated by the statistically significant lower blood vessel count compared with the negative control and the untreated eggs. The hexane partition was observed to be more angiosuppressive compared to the ethyl acetate counterpart. Fractionation by normal phase vacuum column chromatography revealed that the angiosuppressive agents were relatively non-polar. Hexane fraction 4 was the most potent. Further fractionation into subfractions 4.4.2 - 4.4.9 showed decreasing potency which was not comparable to the positive control. Subfractionation showed loss of antiangiogenic activity which may indicate synergistic effects in the parent fraction. Immunohistochemical assays and transmission electron microscopy results support the findings of the CAM assay.


Key words: Antiangiogenic, Ardisia pyramidalis crude extracts, Ardisia pyramidalisfractions.