Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3840

Full Length Research Paper

Caffeic acid and its derivatives as heme oxygenase 1 inducer in Hep G2 cell line

Churdsak Jaikang and Chaiyavat Chaiyasut*
Department of Pharmaceutical Sciences, Faculty of Pharmacy, Chiang Mai University, Chiang Mai, Thailand 50200.
Email: [email protected]

  •  Accepted: 04 May 2010
  •  Published: 18 May 2010

Abstract

Effect of 10, 50 and 100 µg/ml caffeic acid and its esters and amides derivatives on heme oxygenase 1 (HO-1) activity induction in Hep G2 cell line was studied. It was found that caffeic acid ester derivatives could induce HO-1 activity more than amide derivatives. The longer side chain and aromatic side chain affected HO-1 activity. At 10 µg/ml concentration, phenylmethyl 1-(3’, 4’-dihydroxyphenyl) propenate (PMDP) had 2.1-fold more activity when compared with control. At 50 and 100 µg/ml concentrations, phenylethyl 1-(3’, 4’-dihydroxyphenyl) propenate (PEDP) was the highest HO-1 inducer, whose activity was 2.1- and 2.3-fold greater, respectively, when compared with the control group.  Caffeic acid and all derivatives induced HO-1 activity on a higher level than curcumin, positive control.  This study implied that caffeic acid and its derivatives could be used to induce HO-1 to protect tissues against free radicals.

 

Key words: Caffeic acid, caffeic acid derivatives, heme oxygenase 1.