Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3761

Full Length Research Paper

Pharmacokinetics, tissue distribution and oral bioavailability evaluation of isoimperatorin by high-performance liquid chromatography coupled with mass spectrometry

Jiye Zhang1, Fufang Tao2, Yiping Li1, Guangde Yang1*, Zhen Hu1, Yi Li1, Xin Song1and Muxing Li1 and Rong Lin1
1School of Medicine, Xi’an Jiaotong University, Xi’an 710061, P. R. China. 2Shaanxi provincial new drug evaluation center, Xi’an 710065, P. R. China.
Email: [email protected]

  •  Accepted: 07 December 2011
  •  Published: 09 March 2012

Abstract

A simple and specific high performance liquid chromatography coupled with mass spectrometry (HPLC-MS) method was developed to investigate the plasma pharmacokinetics, tissue distribution and oral bioavailability of isoimperatorin (ISOIM), and osthole was employed as internal standard (IS). The separations were performed on a Shim-pack (VP-ODS) C18 analytical column (150 × 2.0 mm, 5 μm) with the column temperature kept at 25°C. The determinations were performed by a quadrupole mass spectrometer operated under selected ion monitoring (SIM) mode. The mobile phase was a mixture of acetonitrile and 0.5% formic acid solution (75:25, v/v) at a flow rate of 0.2 ml/min. The method was linear over the concentration range 2.0 to 5000 ng/ml (correlation coefficients >0.99). The RSDs of the overall intra- and inter-day precisions were less than 3.75%. The recoveries for ISOIM were within the range 82.70 to 97.74%. The pharmacokinetic data were successfully fitted to two-compartment models with first-order absorption. The absolute bioavailability of ISOIM is 68.17%. At 60 min after oral administration, ISOIM was found in all the seven investigated tissues.

 

Key words: Isoimperatorin, pharmacokinetics, tissue distribution, bioavailability, high performance liquid chromatography coupled with mass spectrometry (HPLC-MS).