Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3834

Full Length Research Paper

A simple apparatus for gel staining used in two-dimensional electrophoresis

Shu Haiyan and Tian Baoming*
Department of biology, Zhengzhou University, Henan, China.
Email: [email protected]

  •  Accepted: 12 October 2009
  •  Published: 31 December 2009

Abstract

Two-dimensional electrophoresis was widely used in molecular biology. It was required that all gels be in the same condition. The gels can be controlled in the same condition easily during SDS-PAGE. But no paper reported how to ensure the gels were in the same condition during being stained. When the common protein gel staining plates were used, keeping the gels in the same treatment was impossible. A new apparatus was designed in this paper. The apparatus can ensure all of the gels in the same condition during being stained. Furthermore, the problems such as gels being broken, being covered by others can also be prevented when the new apparatus was used. 

 

Key words: Two-dimensional electrophoresis, gel staining, apparatus