Abstract

The successful extraction of useful DNA from the plants is associated with all extraction steps for molecular techniques used in the next steps such as PCR amplification, digestion and DNA sequencing. Unlike in nonplant DNA extraction protocols, methods need to be adjusted to each species and even to each tissue because of the wide range of primary and secondary metabolites in the plants. Current methods produce degraded and denatured DNA or give extremely poor yields. To overcome these difficulties, we modified available DNA extraction methods. We have obtained higher quality of DNA from corm of Crocusspecies. This extraction technique provides an extraction procedure that can be completed in less than 50 min. This method yielded high-quality and high quantities of DNA. This technique has a potential to be an effective protocol for DNA extraction using corm forCrocus species, and perhaps for other plants, when sufficient young leaf tissue is not available. This could be adopted as a standard method for plants propagated with corm for isolation of DNA for marker analyses.

Key words: DNA extraction, saffron, molecular marker, corm.