Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3834

Full Length Research Paper

Synergistic activity from Hymenaea courbaril L. and Stryphnodendron adstringens (Mart.) Coville against multidrug-resistant bacteria strains

Alan Alex Aleixo
  • Alan Alex Aleixo
  • Laboratorio de Microbiologia, Universidade Federal de Sao Joao del-Rei (UFSJ), Campus Centro Oeste Dona Lindu, Divinopolis, Minas Gerais, Brazil.
  • Google Scholar
Vidyleison Neves Camargos
  • Vidyleison Neves Camargos
  • Laboratorio de Microbiologia, Universidade Federal de Sao Joao del-Rei (UFSJ), Campus Centro Oeste Dona Lindu, Divinopolis, Minas Gerais, Brazil.
  • Google Scholar
Karina Marjorie Silva Herrera
  • Karina Marjorie Silva Herrera
  • Laboratorio de Microbiologia, Universidade Federal de Sao Joao del-Rei (UFSJ), Campus Centro Oeste Dona Lindu, Divinopolis, Minas Gerais, Brazil.
  • Google Scholar
Ana Claudia dos Santos Pereira Andrade
  • Ana Claudia dos Santos Pereira Andrade
  • Laboratorio de Microbiologia, Universidade Federal de Sao Joao del-Rei (UFSJ), Campus Centro Oeste Dona Lindu, Divinopolis, Minas Gerais, Brazil.
  • Google Scholar
Michelli dos Santos
  • Michelli dos Santos
  • Laboratorio de Microbiologia, Universidade Federal de Sao Joao del-Rei (UFSJ), Campus Centro Oeste Dona Lindu, Divinopolis, Minas Gerais, Brazil.
  • Google Scholar
Vivian Correia Miranda
  • Vivian Correia Miranda
  • Laboratorio de Microbiologia, Universidade Federal de Sao Joao del-Rei (UFSJ), Campus Centro Oeste Dona Lindu, Divinopolis, Minas Gerais, Brazil.
  • Google Scholar
Rafaella Souza Carvalho
  • Rafaella Souza Carvalho
  • Laboratorio de Microbiologia, Universidade Federal de Sao Joao del-Rei (UFSJ), Campus Centro Oeste Dona Lindu, Divinopolis, Minas Gerais, Brazil.
  • Google Scholar
Juliana Teixeira de Magalhaes
  • Juliana Teixeira de Magalhaes
  • Laboratorio de Microbiologia, Universidade Federal de Sao Joao del-Rei (UFSJ), Campus Centro Oeste Dona Lindu, Divinopolis, Minas Gerais, Brazil.
  • Google Scholar
Jose Carlos de Magalhaes
  • Jose Carlos de Magalhaes
  • Departamento de Engenharia de Bioprocessos e Biotecnologia, Universidade Federal de Sao Joao del-Rei (UFSJ), Campus Alto Paraopeba, Ouro Branco, Minas Gerais, Brazil.
  • Google Scholar
Luciana Alves Rodrigues dos Santos Lima
  • Luciana Alves Rodrigues dos Santos Lima
  • Laboratorio de Fitoquimica, Universidade Federal de Sao Joao del-Rei (UFSJ), Campus Centro Oeste Dona Lindu, Divinopolis, Minas Gerais, Brazil.
  • Google Scholar
Jaqueline Maria Siqueira Ferreira*
  • Jaqueline Maria Siqueira Ferreira*
  • Laboratorio de Microbiologia, Universidade Federal de Sao Joao del-Rei (UFSJ), Campus Centro Oeste Dona Lindu, Divinopolis, Minas Gerais, Brazil.
  • Google Scholar


  •  Received: 26 June 2014
  •  Accepted: 11 June 2015
  •  Published: 10 July 2015

Abstract

Infections caused by multidrug-resistant bacteria are a problem of public health, turning the search for natural products an alternative to antibiotics of great importance. The aim of this study was to investigate the in vitro antimicrobial activities of Hymenaea courbaril and Stryphnodendron adstringens against bacterial clinical isolates. The crude extracts of both vegetal species in study showed bacteriostatic activity against almost all bacteria evaluated, with minimum inhibitory concentration (MIC) ranging from 125 to 1250 µg/ml. The bacteriostatic activity was observed in all the fractions of H. courbaril and S. adstringens against at least one bacterial strain, except in the fraction dichloromethane of S. adstringens. In regard to bactericidal activity, H. courbaril was active only against E. faecalis, and S. adstringens showed activity against all bacterial strains, except to Enterococcus faecalis. The combination of extracts showed potent synergistic antimicrobial activity, with MIC values of 31.25 µg/ml against Acinetobacter baumannii, Escherichia coli and Staphylococcus aureus. S. adstringens were considered less cytotoxic compared to H. courbaril and the half-maximum cytotoxic concentration (CC50) resulting from the combination of the two plants was 0.0082 ± 3.19 mg/ml. The results showed for the first time the synergic antibacterial activities of H. courbaril and S. adstringens against resistant bacteria, suggesting their potential use to development of new drugs.

 

Key words: Hymenaea courbaril, Stryphnodendron adstringens, antimicrobial activity, synergism, multidrug-resistant bacteria.