Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3835

Full Length Research Paper

Enhanced antibacterial activity in leaf-callus extracts of Alophyllus cobbe L.

H. V. Hegde1*, S. S. Hebbar2, G. R. Hegde2 and S. D. Kholkute1
1Regional Medical Research Centre (ICMR), Nehru Nagar, Belgaum, 590 010, India. 2Department of Botany, Karnatak University, Dharwad, 580 003, India.
Email: [email protected]

  •  Accepted: 17 May 2010
  •  Published: 18 June 2010

Abstract

Antimicrobial activity of crude extracts from the leaves and leaf-callus ofAlopyllus cobbe L. (Sapindaceae) was studied.  Chloroform, acetone, ethanol and water extracts were tested in three different concentrations (250, 200 and 150 mg/disk) against two gram (+)ve and two  gram (-)ve strains of bacteria namely Bacillus subtilis,  Streptococcus faecalis, Escherichia coli andPseudomonas aeruginosa. Streptomycin was the control, while disk impregnated with DMF was used as blank. Acetone extract showed maximum activity in both leaf and leaf-callus extracts. It is also found that the zone of inhibition was much higher in callus extracts, when compared to that of leaf extracts.

 

Key words: Alophyllus cobbe, antimicrobial activity, callus extract, leaf extract