Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3778

Full Length Research Paper

Hydroxytyrosol supplementation inhibits oxidative DNA damage, suppresses protein-lipid oxidation and modulates antioxidant enzymes in human peripheral blood lymphocytes

Bochra Gargouri1*, Riadh ben Mansour1, Malek Mseddi Jmal1, Rihab Nasr Boujelben1, Noureddine Allouch2, Saloua Lassoued1 and Khaled Hamden2
1Unité de Biotechnologie et Pathologies, Institut Supérieur de Biotechnologie de Sfax, Tunisie. 2Unité de Physiologie Animale, Faculté des Sciences de Sfax, Tunisie.
Email: [email protected]

  •  Accepted: 07 June 2011
  •  Published: 23 September 2011

Abstract

We investigated the oxidative modifications of DNA, proteins and antioxidants enzymes activities, in peripheral blood lymphocytes (PBL) treated with H2O2 in the presence or absence of hydroxytyrosol. Interestingly, we found a potential protection of DNA from fragmentation after hydroxytyrosol supplementation. In addition, this study showed a prospective protective action of hydroxytyrosol of PBL from the attack of reactive oxygen species. In fact, a considerable increase of superoxide dismutase and catalase activities by 76.92 and 21.67%, respectively was showed after 20 µg/ml hydroxytyrosol treatment. Moreover, 20 µg/ml of hydroxytyrosol significantly decreased the lipid peroxidation and protein oxidation rates by 97.89 and 18.5% respectivelyIn conclusion, the present study reveals the efficacy of hydroxytyrosol in the protection of PBL of DNA fragmentation and oxidative stress induced by H2O2 treatment, which may be attributed to its anti-oxidant potential.

 

Key words: Hydroxytyrosol, thiol, lymphocytes, conjugated dienes, malondialdehyde, superoxide dismutase, catalase.