Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3835

Full Length Research Paper

Phytochemical screening, total phenolic content and antioxidant activity of some plants from Brazilian flora

João da Rocha Lins Neto
  • João da Rocha Lins Neto
  • Departamento de Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Pernambuco, Recife, Pernambuco, Brazil.
  • Google Scholar
Amanda Dias de Araújo Uchôa
  • Amanda Dias de Araújo Uchôa
  • Departamento de Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Pernambuco, Recife, Pernambuco, Brazil.
  • Google Scholar
Priscila Andrade de Moura
  • Priscila Andrade de Moura
  • Departamento de Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Pernambuco, Recife, Pernambuco, Brazil.
  • Google Scholar
Clovis Macêdo Bezerra Filho
  • Clovis Macêdo Bezerra Filho
  • Departamento de Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Pernambuco, Recife, Pernambuco, Brazil.
  • Google Scholar
Juciara Carneiro Gouveia Tenório
  • Juciara Carneiro Gouveia Tenório
  • Departamento de Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Pernambuco, Recife, Pernambuco, Brazil.
  • Google Scholar
Alexandre Gomes da Silva
  • Alexandre Gomes da Silva
  • Núcleo de Bioprospecção e Conservação da Caatinga, Instituto Nacional do Semiárido/Ministério da Ciência, Tecnologia e Inovação (INSA/MCTI), Campina Grande, Paraíba, Brazil.
  • Google Scholar
Rafael Matos Ximenes
  • Rafael Matos Ximenes
  • Departamento de Antibióticos, Centro de Ciências Biológicas, Universidade Federal de Pernambuco, Recife, Pernambuco, Brazil.
  • Google Scholar
Márcia Vanusa da Silva
  • Márcia Vanusa da Silva
  • Departamento de Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Pernambuco, Recife, Pernambuco, Brazil.
  • Google Scholar
Maria Tereza dos Santos Correia
  • Maria Tereza dos Santos Correia
  • Departamento de Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Pernambuco, Recife, Pernambuco, Brazil.
  • Google Scholar


  •  Received: 19 October 2015
  •  Accepted: 04 April 2016
  •  Published: 17 July 2016

Abstract

The present study evaluated the total phenolic and flavonoid content as well as the antioxidant activity of methanolic leaf extracts of five plants from Brazilian flora: Abarema cochliacarpos, Croton corchoropsis, Myroxylon peruiferum, Stryphnodendron pulcherrimum and Tanaecium cyrtanthum by 2,2’-azino-bis- (3-ethylbenzothiazoline-6-sulfonate) (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH) and total antioxidant capacity assays. A thin layer chromatography analysis of all plant extracts has also been performed and it showed the presence of different types of secondary metabolites, namely saponins, phenylpropanoids and flavonoids. Among the studied plants, A. cochliacarpos and S. pulcherrimum showed considerable antioxidant radical scavenging activity on all the tested assays and they also exhibited substantial amounts of phenolic compounds. In addition, a positive correlation was found between total phenols and both ABTS radical scavenging activity and total antioxidant capacity assays, thus indicating the major role of phenols on the antioxidant activity of these plants. To the best of the authors’ knowledge, this is the first approach where the phenolic content and antioxidant activity of A. cochliacarpos, C. corchoropsis, M. peruiferum, S. pulcherrimum and T. xanthophyllum were explored.

Key words: Brazilian medicinal plants, 2, 2-diphenyl-1-picrylhydrazyl (DPPH), 2,2’-azino-bis-(3-ethylbenzothiazoline-6-sulfonate) (ABTS), flavonoid content, phenolic content.