Mentha spicata is an herb with several biological activity. The study assessed the cytotoxicity activity in vitro of ethanolic, methanolic and aqueous extracts from Mentha spicata against human breast cancer cells lines (MDA-MB-231) using MTT assay and cultured in DMEM with 10% gentamicin. In the presence of different extracts (Aqueous, ethanolic and methanolic), the cells were incubated at 24 and 48 hours with controls. The cell proliferation was assessed with MTT assays for evaluation of cells viability and lethal dose. The optical density was determined in an ELISA multi plate reader (ELX800 BIOTEK ELISA Reader). In addition, antibacterial effect of M. spicata was assessed with disk diffusion and Kirby-Bauer diffusion method. Thus, Mentha spicata could have potential anti-tumor and antibacterial activity. In order to prevent the probable side effects, studies of Mentha spicata extracts on normal cells and tissues are recommended. In vivo studies as well as identification of effective components of M. spicata with anti-cancer activity and their mechanism mediating the anti-cancer reaction. This could be a step forward in the research of a new anti-cancer therapeutic agents
Keywords: Mentha spicata, extracts, anti-tumor potential, antibacterial activity