Journal of
Plant Breeding and Crop Science

  • Abbreviation: J. Plant Breed. Crop Sci.
  • Language: English
  • ISSN: 2006-9758
  • DOI: 10.5897/JPBCS
  • Start Year: 2009
  • Published Articles: 409

Full Length Research Paper

Structural features of a cytoplasmic male sterility source from Helianthus resinosus, CMS RES1

F. Ardila1,2, M.  M. Echeverría 1,3*, R. Rios2 and R. H. Rodríguez3
  1Ex Aequo 2Instituto de Genética CICV y A, CNIA, INTA. C.C. 25 (1712) Castelar, Buenos Aires, Argentina. 3Unidad Integrada: Facultad de Ciencias Agrarias, Universidad Nacional de Mar del Plata - Estación Experimental INTA Balcarce. C.C. 276 (7620) Balcarce, Buenos Aires, Argentina.
Email: [email protected]

  •  Accepted: 31 May 2010
  •  Published: 31 July 2010

Abstract

 

Comparisons in the structural organization of mitochondrial DNA between a cytoplasmic male sterility source from Helianthus resinosus (CMS RES1) and HA89 fertile line have been performed surrounding coxIII and atp9 genes. Differences have also been recognized by PCR analyses between HA89 (CMS RES1) and either HA89 (CMS PET1) or HA89 (CMS MAX1), indicating that the molecular basis of their sterility mechanism is different. To be specific, RES1 lacks orfH522, which is present in the mitochondrial DNA of PET1 and MAX1.

 

Key words: Helianthus annuusHelianthus resinosus, sunflower, cytoplasmic male sterility, CMS, coxIII, atp9.