Sinorhizobium freddi R0132, which forms nitrogen fixing nodules in association with Vigna radatia L. and fix nitrogen symbiotically, was the bioinoculant used in this study. Drug resistant S. freddi R0132::Tn5 remained constant at 40°C higher levels temperature for about 35 days (5 weeks) from the date of inoculation in solarzied and autoclaved biomanure VC-MA. The Tn5 mutagenesis was carried by the random transposon mutagenesis. The population of drug resistant S. freddiR0132::Tn5 in these treatments was significantly higher as compared to control. The present study attempted to evaluate the role of the said bio-manure VC-MA as carrier for survival of improved drug resistant S. freddi R0132::Tn5 in all the carrier material tested as compared to sample at higher (37 - 40°C) temperature, and higher population of drug resistant S. freddi R0132::Tn5 were observed when stored at relatively lower temperature (28°C). It is therefore not recommended to store the mass culture of biofertilizers particularly at higher temperature.
Key words: Rhizobium, transposon Tn5 mutagenesis, biomanure.
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