Journal of
Plant Breeding and Crop Science

  • Abbreviation: J. Plant Breed. Crop Sci.
  • Language: English
  • ISSN: 2006-9758
  • DOI: 10.5897/JPBCS
  • Start Year: 2009
  • Published Articles: 409

Full Length Research Paper

Genetic diversity and inbreeder specie of Andrographis paniculata (Burm. f.) Nees by randomly amplified polymorphic deoxyribonucleic acid (RAPD) and floral architecture analysis

Parwat Wijarat1,2*, Vichien Keeratinijakal3, Theerayut Toojinda4, Apichart Vanavichit4,5 and Somvong Tragoonrung6
  1Genetic Engineering Program, Kasetsart University, Bangkok 10900, Thailand. 2Agricultural Science Program, Mahidol University, Nakhon Sawan Campus, Nakhon Sawan 60000, Thailand. 3Department of Agronomy, Faculty of Agriculture, Kasetsart University, Bangkok 10900, Thailand. 4Rice Gene Discovery Unit, Rice Science Center and DNA Technology Laboratory, National Center for Genetic Engineering and Biotechnology, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140, Thailand. 5Department of Agronomy, Faculty of Agriculture, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140, Thailand. 6Genome Institute, National Center for Genetic Engineering and Biotechnology, Thailand Science Park, Pathumthani 12120, Thailand.
Email: [email protected]

  •  Accepted: 07 October 2011
  •  Published: 15 October 2011

Abstract

 

“Fha-Tha-Laai-Joan” (Andrographis paniculata (Burm. f.) Nees) is a medicinal plant that is, widely used in Thailand and some other countries for therapeutic value, but the lack of their genetic information may cause confusion in its utilization. The randomly amplified polymorphic DNA (RAPD) markers were used to identify and elucidate the phylogenetic relationships among 58 accessions of A. paniculata which were collected throughout Thailand. Of thirty random primers tested, only eight primers generated a total of 66 bands, of which 26 bands were polymorphic, with an average of 8.25 bands per primer pair. The phylogenetic tree derived from RAPD data showed that A. paniculata were divided into only one group in which the high similarity values were between 0.81-1.00. The results obtained  indicated that the distributions of A. paniculataamong each region are likely to belong to the same variety and are relatively undifferentiated across a large geographic range. The floral architecture and reproductive habitat study of A. paniculata revealed the same results as of RAPD markers analysis. This species is thus hermaphroditic and acts as habitual inbreeder.

 

Key words:  Andrographis paniculata, randomly amplified polymorphic DNA (RAPD), floral architecture, monomorphic, Thailand.

Abbreviation

RAPD, Randomly amplified polymorphic deoxyribonucleic acid; TTM,Thai traditional medicine; HIV, human immunodeficiency virus; CTAB, cetyltrimethyl ammonium bromide; EDTA, ethylene  diamine  tetraacetic  acid; UV, ultraviolet; PCR,polymerase chain reaction; UPGMA, unweighted pair-group method with arithmetic averages; SSCP, single-strand conformation polymorphism; SSR, simple sequence repeat; AFLP, amplified fragment length polymorphism; dNTPs, deoxynucleotide triphosphates; ISSR, inter simple sequence repeat.