Journal of
Plant Breeding and Crop Science

  • Abbreviation: J. Plant Breed. Crop Sci.
  • Language: English
  • ISSN: 2006-9758
  • DOI: 10.5897/JPBCS
  • Start Year: 2009
  • Published Articles: 409

Full Length Research Paper

Genetic diversity of Cymbidium kanran detected by Polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) markers

JIAN Li
  • JIAN Li
  • Department of Environment and Life Science, BiJie University, Guizhou, 551700, China.
  • Google Scholar
ZHU Li-quan
  • ZHU Li-quan
  • College of Agronomy and Biotechnology, Southwest University, Chongqing, 4007151, China.
  • Google Scholar


  •  Accepted: 02 August 2013
  •  Published: 31 August 2013

Abstract

 

 

Fifty-four Cymbidium kanran cultivars from China, Japan and South Korea were examined and analyzed by using polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) markers to determine their molecular diversity and relationships. In chloroplast (cp) PCR-RFLP analyses, genetic differences were revealed in 4 of 6 primer sets (66.67%) and 19 of 72 primer-enzyme combinations (26.38%), 116 polymorphic bands were detected. For mitochondrion (mt) PCR-RFLP markers, genetic differences were revealed in 2 of 8 primer sets (25%) and 55 polymorphic bands (53.49%) were detected with 7 restriction primer-enzyme combinations. According to the PCR-RFLP marker profiling data, all the cultivars were classified into four groups. The group 1 has included three subclusters that two of them were generally consistent with geography classification. Subcluster 1 and Subcluster 2 were mainly composed of Chinese cultivars. Comparably, Subcluster 3 was composed of two cultivars which originated from Japan separately. Group 2 comprised Huangchengzhiyue and Xiongnu originated from Japan. Group 3 was included in seven Chinese cultivars and five Japanese cultivars. Group 4 was composed of eight Chinese cultivars and four Japanese cultivars. Therefore, we demonstrated that the PCR-RFLP technique could provide a powerful tool for cultivar identification and establishment of genetic relationships of cultivars in cymbidium kanran.

 

Key words: Cymbidium kanran, chloroplast (cp) polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP), mitochondrion (mt) PCR-RFLP.