Journal of
Parasitology and Vector Biology

  • Abbreviation: J. Parasitol. Vector Biol.
  • Language: English
  • ISSN: 2141-2510
  • DOI: 10.5897/JPVB
  • Start Year: 2009
  • Published Articles: 202

Full Length Research Paper

Development and evaluation of lateral flow test for the detection of trypanosomes in tsetse flies

Enyaru C. K. John
  • Enyaru C. K. John
  • Department of Biochemistry and Sports Sciences, Makerere University, P. O. Box 7062, Kampala, Uganda.
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Njuguna James
  • Njuguna James
  • Astel Diagnostics, Uganda.
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Alibu Vincent Pius
  • Alibu Vincent Pius
  • Department of Biochemistry and Sports Sciences, Makerere University, P. O. Box 7062, Kampala, Uganda.
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Matovu Enock
  • Matovu Enock
  • College of Veterinary Medicine and Biosafety, Makerere University, P. O. Box 7062, Kampala, Uganda.
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Malele I. Imna
  • Malele I. Imna
  • Vector and Vector Borne Diseases Institute, Tanga, Tanzania.
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Chisi E. John
  • Chisi E. John
  • College of Medicine, University of Malawi, Private bag 360, Blantyre, Malawi.
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Mbongo Nicolas
  • Mbongo Nicolas
  • Laboratoire national de Sante Publique, Brazzaville, Congo.
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Mansinsa Philemon
  • Mansinsa Philemon
  • National Program of Human African Trypanosomosis, Kinshasa, Congo.
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Intisar E. l. Rayah
  • Intisar E. l. Rayah
  • Tropical Medicine Research Institute, Khartoum, Sudan.
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Mohammed Yassir
  • Mohammed Yassir
  • Tropical Medicine Research Institute, Khartoum, Sudan.
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Mubarak Mustafa
  • Mubarak Mustafa
  • Tropical Medicine Research Institute, Khartoum, Sudan.
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Ochi Erneo
  • Ochi Erneo
  • Ministry of Animal Resources and Fisheries, Juba, South Sudan.
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Nantulya Vinand
  • Nantulya Vinand
  • Astel Diagnostics, Uganda.
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  •  Received: 16 October 2014
  •  Accepted: 22 December 2014
  •  Published: 31 December 2014

References

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Bosompem KM, Assoku RKG, Nantulya VM (1996). Differentiation between culture-derived insect stages of T. brucei, T. vivax, T. congolense and T.simiae using monoclonal antibody-based dot-ELISA. Parasitology 112:59-66.
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Msangi AR, Whitaker CJ, Lehane MJ (1998). Factors influencing the prevalence of trypanosome infection of Glossina pallidipes on the Ruvu flood plain of Eastern Tanzania. Acta Trop. 70:143-155.
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Milligan PJ, Maudlin I, Welburn SC (1995). Trypanozoon: infectivity to humans is linked to reduced transmissibility in tsetse. II. Genetic mechanisms. Exp. Parasitol. 81:409-415.
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Nantulya VM, Musoke AJ, Rurangirwa FR, Saigar N, Minja SH (1987). Monoclonal antibodies that distinguish Trypanosoma congolense, T. vivax and T. brucei. Parasite Immunol. 8:421-431.
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Okoth JO, Kapaata R (1986). Trypanosome infection rates in Glossina fuscipes fuscipes Newst. in the Busoga sleeping sickness focus, Uganda. Ann. Trop. Med. Parasitol. 80:459-61.
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Welburn SC, Maudlin I, Milligan PJ (1995). Trypanozoon: infectivity to humans is linked to reduced transmissibility in tsetse. I. Comparison of human serum-resistant and human serum-sensitive field isolates. Exp. Parasitol 81:404-408.
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Wery M, Wery-Paskoff S, Van Wettere N (1970). The diagnosis of human African trypanosomiasis (T. gambiesnes) by the use of fluorescent antibody test. 1. Standardization of an easy technique to be used in mass surveys. Ann. Soc. Belg. Med. Trop. 50 (5):613-634.

 

World Health Organization (WHO) (2014). WHO fact sheet on Human African trypanosomiasis. Fact Sheet No 259.