Journal of
Stored Products and Postharvest Research

  • Abbreviation: J. Stored Prod. Postharvest Res.
  • Language: English
  • ISSN: 2141-6567
  • DOI: 10.5897/JSPPR
  • Start Year: 2010
  • Published Articles: 147

Full Length Research Paper

Response of cucurbitacin B concentration in Nemafric-BL phytonematicide to increasing storage period

Kagiso Given Shadung
  • Kagiso Given Shadung
  • Limpopo Agro-Food Technology Station, University of Limpopo, Private Bag X 1106, Sovenga 0727, South Africa
  • Google Scholar
Phatu William Mashela
  • Phatu William Mashela
  • Green Technologies Research Centre, University of Limpopo, Private Bag X 1106, Sovenga 0727, South Africa
  • Google Scholar
Maboko Samuel Mphosi
  • Maboko Samuel Mphosi
  • Limpopo Agro-Food Technology Station, University of Limpopo, Private Bag X 1106, Sovenga 0727, South Africa
  • Google Scholar


  •  Received: 31 December 2015
  •  Accepted: 28 February 2016
  •  Published: 31 March 2016

Abstract

Nemafric-BL phytonematicide, produced from fermented fruit of wild watermelon (Cucumis africanus), had been consistent in suppressing root-knot (Meloidogyne species) nematode population densities. However, due to the biological nature of Nemafric-BL phytonematicide, quality could be compromised during increasing storage period. A study was, therefore, conducted to determine the response of cucurbitacin B concentration in Nemafric-BL phytonematicide over a six-month storage period. The product, in 50 ml hermetically-sealed plastic containers, was stored in a dark room at room temperature (±25°C), with 10 samples being analysed monthly for cucurbitacin B concentrations using Shimadzu High Performance Liquid Chromatography (HPLC). Cucurbitacin concentrations over increasing storage period exhibited density-dependent growth patterns, which were characterised by increases in cucurbitacin B during the initial period of storage and followed by gradual decreases. Relative to the initial storage time (T0), at the end of the storage period, cucurbitacin B concentration was still more than three-hundred times that at T0, suggesting that the product was still suitable for use in managing nematode numbers.

 

Key words: Cucumis africanus, effective microorganisms, product quality, phytonematicide, shelf-life.