Journal of
Veterinary Medicine and Animal Health

  • Abbreviation: J. Vet. Med. Anim. Health
  • Language: English
  • ISSN: 2141-2529
  • DOI: 10.5897/JVMAH
  • Start Year: 2009
  • Published Articles: 347

Full Length Research Paper

Resistance to 3rd generation cephalosporin of Escherichia coli isolated from the feces of healthy broilers chickens in Algeria

Moustafa Sellah*
  • Moustafa Sellah*
  • Laboratoire antibiotiques, antifongiques: physico-chimie, synthese et activite biologiques (LAPSAB), faculte des sciences de la nature et de la vie et des sciences de la terre et de lunivers, Universite Abou Bekr Belkaid-Tlemcen, Tlemcen 13000, Algerie.
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Mourad Drissi
  • Mourad Drissi
  • Laboratoire antibiotiques, antifongiques: physico-chimie, synthese et activite biologiques (LAPSAB), faculte des sciences de la nature et de la vie et des sciences de la terre et de lunivers, Universite Abou Bekr Belkaid-Tlemcen, Tlemcen 13000, Algerie.
  • Google Scholar


  •  Received: 14 May 2015
  •  Accepted: 27 July 2015
  •  Published: 31 August 2015

Abstract

High resistance of Escherichia coli have been demonstrated to 3rd generation cephalosporin in livestock, especially in broiler chickens; however, data on emission sources of these bacteria into Algeria are still rare. From January to March 2014, a preliminary epidemiological study of E. coli contamination in healthy broiler chicken flocks was carried out in the regions of Tlemcen, Algeria. 21 E. coli resistant isolates were examined to 3rd generation cephalosporin antibiotics (ceftriaxone, ceftazidime and cefotaxime). The antimicrobial susceptibility was determined by disk diffusion, and the MICs were determined by agar dilution method with Antibiogram Committee of the French Society for Microbiology (CA-SFM) 2013 guidelines. All strains were resistant to ceftriaxone and cefotaxime referring to CA-SFM, Antibiogram Committee of the French Society for Microbiology (EUCAST), and Clinical and Laboratory Standards Institute (CLSI). However, the resistance rate of ceftazidime is different according to the breakpoints criteria used; the susceptibility result of CA-SFM and EUCAST is similar for each farm. Farm B 50% of E. coli was resistant and 50% was susceptible, and 21% was susceptible and 79% was resistant for the farm C. However, comparing these two with CLSI, all strains were susceptible to ceftazidime.
 
Key words: Escherichia coli, antimicrobial resistance, 3rd generation cephalosporin, feces, broilers chicken.