Journal of
Veterinary Medicine and Animal Health

  • Abbreviation: J. Vet. Med. Anim. Health
  • Language: English
  • ISSN: 2141-2529
  • DOI: 10.5897/JVMAH
  • Start Year: 2009
  • Published Articles: 388

Full Length Research Paper

Antiviral activities of ellagitannins against bovine herpesvirus-1, suid alphaherpesvirus-1 and caprine herpesvirus-1

Neli Vilhelmova-Ilieva
  • Neli Vilhelmova-Ilieva
  • Department of Virology, The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, 26 G. Bonchev Str., BG-1113 Sofia, Bulgaria.
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Ivo Sirakov
  • Ivo Sirakov
  • Department of Medical Microbiology, Medical Faculty, Medical University of Sofia, 2 Zdrave Street, BG-1431 Sofia, Bulgaria.
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Remi Jacquet
  • Remi Jacquet
  • Universitéde Bordeaux, Institut des Sciences Moléculaires (CNRS-UMR 5255), Institut Européen de Chimie et Biologie, 2 rue Robert Escarpit, 33607 Pessac Cedex, France.
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Stephane Quideau
  • Stephane Quideau
  • Universitéde Bordeaux, Institut des Sciences Moléculaires (CNRS-UMR 5255), Institut Européen de Chimie et Biologie, 2 rue Robert Escarpit, 33607 Pessac Cedex, France.
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Angel S. Galabov
  • Angel S. Galabov
  • Department of Virology, The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, 26 G. Bonchev Str., BG-1113 Sofia, Bulgaria.
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  •  Received: 26 June 2020
  •  Accepted: 23 July 2020
  •  Published: 31 October 2020

Abstract

In vitro antiviral activity of three ellagitannins - castalagin, vescalagin and grandinin was investigated against replication of three of the most common herpes viruses infecting animals, namely bovine herpesvirus-1 (BoHV-1), suid alphaherpesvirus-1 (SuHV-1), and caprine herpesvirus-1 (CapHV-1). The cytopathic effect inhibition test via the neutral red uptake assay in Madin-Darby bovine kidney (MDBK) cell culture was applied. The highest activity was demonstrated by castalagin followed by vescalagin against replication of SuHV-1, strain A2, with selectivity index values of 336.8 and 309, respectively, which are close to that of acyclovir (SI = 540). The activity of grandinin against the replication of SuHV-1 A2 strain (SI = 40.8) as well as the activity of all three substances against the replication of BoHV-1 strain K22 was lower but still significant (castalagin SI = 45; vescalagin SI = 42.5; grandinin SI = 32.3). Against the E/CH strain of CapHV-1, the three ellagitannins showed a moderate to weak activity: Castalagin SI = 19.3, vescalagin SI = 18.8, grandinin SI = 11.8. The results obtained characterize compounds tested as perspective antivirals.

Key words: bovine herpesvirus-1, suid alphaherpesvirus-1, caprine herpesvirus-1, ellagitannins, alpha acyclovir, antiviral activity.