Journal of
Veterinary Medicine and Animal Health

  • Abbreviation: J. Vet. Med. Anim. Health
  • Language: English
  • ISSN: 2141-2529
  • DOI: 10.5897/JVMAH
  • Start Year: 2009
  • Published Articles: 328

Full Length Research Paper

Toxicological assessment of Cryptolepis sanguinolenta for possible use in veterinary medicine

C. Ansah
  • C. Ansah
  • Department of Pharmacology, College of Health Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
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H. R. Otsyina
  • H. R. Otsyina
  • CSIR- Animal Research Institute, P.O. Box AH 20, Achimota, Accra, Ghana.
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M. Duwiejua
  • M. Duwiejua
  • Department of Pharmacology, College of Health Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
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E. Woode
  • E. Woode
  • Department of Pharmacology, College of Health Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
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F. A. Aboagye
  • F. A. Aboagye
  • Centre for Scientific Research into Plant Medicine, Mampong-Akwapim, Ghana
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K. G. Aning
  • K. G. Aning
  • College of Agriculture and Consumer Sciences, University of Ghana, Legon, Ghana.
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  • Article Number - C4F05EE834
  • Vol.1(1), pp. 11-016, July 2009
  •  Published: 30 July 2009

Abstract

 

Acute and sub-acute oral toxicity assessment of the aqueous root extract ofCryptolepis sanguinolenta was studied in Sprague Dawley rats for possible use as animal medication. The extract (250 - 3000 mg/kg, p.o) was administered daily for a period of 72 h and (500 - 2000 mg/kg, p.o) for 14 days for acute and sub-acute studies respectively.  Acute administration of the extract did not produce any physiological and behavioural changes. In the subacute toxicity studies however, a dose-dependent increase in the number of platelets (from a vehicle-treated control value of 353.00 ± 49.40 - 958.00 ± 42.50 in animals treated with 2000 mg/kg) was observed.  Granulocyte number also increased dose-dependently (0.77±0.15 - 3.70±0.20) from the vehicle-treated control to the group that received 2000 mg/kg, indicating possible inflammation.  Central nervous system toxicity and marginal enlargement of liver and kidney were evident in the 2000 mg/kg treated group. These findings however did not correlate with the biochemical and histopathological studies as no pathological changes occurred in the renal or hepato-biliary systems. The present results suggest that the aqueous root extract of C. sanguinolenta < 500 mg/kg orally is generally safe. However, caution should be taken with doses > 500 mg/kg as these may induce thrombocytosis, inflammation and central nervous system toxicity.

 

Key words:  Sub-acute toxicity, rats, Cryptolepis sanguinolenta extract.