Journal of
Veterinary Medicine and Animal Health

  • Abbreviation: J. Vet. Med. Anim. Health
  • Language: English
  • ISSN: 2141-2529
  • DOI: 10.5897/JVMAH
  • Start Year: 2009
  • Published Articles: 400

Full Length Research Paper

Evaluation of two Immune-Enzimatic Assays (ELISAs) for detecting Anti-Map anti-bodies in domestic ruminants

Marco Antonio Santillan-Flores
  • Marco Antonio Santillan-Flores
  • CENID-SAI INIFAP, Carretera Mexico Toluca Km. 15.5, Col. Palo alto, Delegacion Cuajimalpa C.P. 05510, México.
  • Google Scholar
Dionicio Cordova-Lopez
  • Dionicio Cordova-Lopez
  • CENID-SAI INIFAP, Carretera Mexico Toluca Km. 15.5, Col. Palo alto, Delegacion Cuajimalpa C.P. 05510, México.
  • Google Scholar
Marisela Leal- Hernandez
  • Marisela Leal- Hernandez
  • CENID-SAI INIFAP, Carretera Mexico Toluca Km. 15.5, Col. Palo alto, Delegacion Cuajimalpa C.P. 05510, México.
  • Google Scholar
Fernando Ceron-Tellez
  • Fernando Ceron-Tellez
  • CENID-SAI INIFAP, Carretera Mexico Toluca Km. 15.5, Col. Palo alto, Delegacion Cuajimalpa C.P. 05510, México.
  • Google Scholar
Raquel Torres-Velez
  • Raquel Torres-Velez
  • FMVZ-FES Cuautitlan, Universidad Nacional Autónoma de México.
  • Google Scholar
Luis Armando Contreras-Mendez
  • Luis Armando Contreras-Mendez
  • Instituto Tecnológico de Sonora, México.
  • Google Scholar


  •  Received: 16 September 2022
  •  Accepted: 24 October 2022
  •  Published: 30 November 2022

Abstract

The objective of the work was to evaluate two ELISA’s for the detection of anti-Map antibodies. 460 serum and fecal samples of bovines (n=267), ovines (n=140), and goats (n=53) were evaluated. Serums were used for detecting anti-Map antibodies using two different ELISAs, one in-house ELISA made with low-molecular weight proteins (<100 kD) from the antigen ultra-filtrate of Map strain 3065, and a commercial ELISA for paratuberculosis diagnostics. DNA was extracted from faeces to carry out PCR-N IS900. Sensitivity (Se), specificity (E) and Kappa (K) were calculated to estimate concordance between tests. The in-house ELISA made from low-weight map proteins showed a Se of 87%, E of 96% and K of 0.85, while the commercial ELISA showed Se=55%, E=97% and K=0.576, when compared to the PCR-N IS900 test. When comparing both ELISAs, Se was 50%, E was 97%, and K= 0.393. The in-house ELISA made from <100 kD proteins had a better Se and E than the commercial ELISA; concordance between them was low. Low-weight proteins (<100 kD) obtained from the antigen ultra-filtrate of Map strain 3065 are a good candidate for using as antigen in ELISA for the diagnosis of Johne’s disease

Key words: Paratuberculosis, ELISA, diagnostic.