Journal of
Veterinary Medicine and Animal Health

  • Abbreviation: J. Vet. Med. Anim. Health
  • Language: English
  • ISSN: 2141-2529
  • DOI: 10.5897/JVMAH
  • Start Year: 2009
  • Published Articles: 348

Full Length Research Paper

Antibiotic sensitivity and sodium chloride susceptibility patterns of Flavobacterium columnare isolated from clinical columnaris in cultured Clarias gariepinus

Oladosu Gbolahanmi Akinola
  • Oladosu Gbolahanmi Akinola
  • Department of Veterinary Medicine, Faculty of Veterinary Medicine, University of Ibadan, Oyo State, Nigeria.
  • Google Scholar
Oladosu Oluwatomilola Olakunbi
  • Oladosu Oluwatomilola Olakunbi
  • Department of Agricultural Science, Faculty of Vocational and Technical Education, Ignatius Ajuru University of Education, Port-Harcourt, Rivers State, Nigeria.
  • Google Scholar


  •  Received: 11 September 2018
  •  Accepted: 10 December 2018
  •  Published: 31 March 2019

Abstract

Antimicrobial resistance is a global concern, especially with the backdrop of the development of possible environmental and public health hazards. Flavobacterium columnare has been observed to be multi-drug resistant but highly susceptible to sodium chloride, hence the need for susceptibility profiling. Therapeutic efficacy of commonly used antibiotics and sodium chloride was tested in-vitro against four isolates of F. columnare using the disc diffusion and the pour plate methods, respectively. Comparative in-vivo testing was performed on experimentally infected Clarias gariepinus juveniles in 11 groups of 15 fish each. Nine groups were treated with 1.0, 2.0 and 3.0% NaCl for 5, 10 and 30 min each. A tenth group was exposed to 25 mg L-1 ciprofloxacin for 1 h by immersion, while the eleventh group was not treated (positive control). Growth inhibition was observed to be highest with ciprofloxacin followed by ofloxacin and tetracycline in that order, and also in all NaCl concentrations. There was no mortality in the infected fish groups treated with 25 mg L-1 ciprofloxacin, 1% NaCl, and 2 to 3% NaCl for 5 and 10 min only. However, 46.7 ± 9.4 % mortality observed in 3 % NaCl treatment for 30 min was significantly higher than the 23.3 ± 4.6% observed in 2 % NaCl for 30 min, but not significantly different from the positive control with 36.7±4.7 % mortality. Since short duration sodium chloride bath was found to be effective in the control of columnaris disease, 1% salt disinfection of fry and fingerling stock for 30 min could be incorporated into routine management in catfish hatchery, without the fear of environmental or public health hazards.

Key words:   Antibiogram, columnaris disease, sodium chloride, susceptibility.