Journal of
Veterinary Medicine and Animal Health

  • Abbreviation: J. Vet. Med. Anim. Health
  • Language: English
  • ISSN: 2141-2529
  • DOI: 10.5897/JVMAH
  • Start Year: 2009
  • Published Articles: 348

Full Length Research Paper

Serologic detection of Avian influenza H5 antibodies using a competitive enzyme-linked immunosorbent assay (ELISA)

Ming Yang
  • Ming Yang
  • National Centre for Foreign Animal Disease, 1015 Arlington Street, Winnipeg, Manitoba, Canada R3E 3M4, Canada.Department of Immunology, University of Manitoba, Winnipeg, Manitoba, Canada.
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Alfonso Clavijo
  • Alfonso Clavijo
  • National Centre for Foreign Animal Disease, 1015 Arlington Street, Winnipeg, Manitoba, Canada R3E 3M4, Canada.Texas Veterinary Medical Diagnostic Laboratory, 1 Sippel Road, College Station, USA TX 77843, USA.
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John Pasick
  • John Pasick
  • National Centre for Foreign Animal Disease, 1015 Arlington Street, Winnipeg, Manitoba, Canada R3E 3M4, Canada.
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Tim Salo
  • Tim Salo
  • National Centre for Foreign Animal Disease, 1015 Arlington Street, Winnipeg, Manitoba, Canada R3E 3M4, Canada.
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Zhiliang Wang
  • Zhiliang Wang
  • China Animal Health and Epidemiology Center, 369 Nanjing Road, Qingdao, Shandong 266032, China.
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Yanling Zhao
  • Yanling Zhao
  • China Animal Health and Epidemiology Center, 369 Nanjing Road, Qingdao, Shandong 266032, China.
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Dongxia Zheng
  • Dongxia Zheng
  • China Animal Health and Epidemiology Center, 369 Nanjing Road, Qingdao, Shandong 266032, China.
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Yohannes Berhane
  • Yohannes Berhane
  • National Centre for Foreign Animal Disease, 1015 Arlington Street, Winnipeg, Manitoba, Canada R3E 3M4, Canada.
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  • Article Number - FD59A3A3558
  • Vol.3(4), pp. 56-61, August 2011
  •  Accepted: 27 June 2011
  •  Published: 31 August 2011

Abstract

A competitive enzyme-linked immunosorbent assay (cELISA) for Avian influenza (AI) H5 specific antibody detection was previously developed. In this study, additional serum samples from infected and vaccinated birds were tested and the results were compared to other serological tests. Using the samples from experimentally infected chickens, the H5 cELISA was shown to be comparable to other serological assays. Using samples from free-living blue-winged teals and vaccinated birds, a correlation was found between the H5 cELISA and the hemagglutination-inhibition (HI) assay. In conclusion, the H5 cELISA will be a useful tool for the serological diagnosis, surveillance of AI H5 infections, and for measuring of protective antibody levels.

Key words: Avian influenza, serologic diagnosis, competitive enzyme-linked immunosorbent assay, protective antibody.