Salmonella, a bacterium from the Enterobacteriaceae family, is the source of the bacterial infection known as salmonellosis. Between November 2019 to May 2019, a cross-sectional study was conducted to isolate Salmonella from poultry farms in the towns of Bishoftu and Adama and to ascertain the frequency of the isolates' antimicrobial susceptibility. Using the ISO, 2002 standard methods, 384 samples in total were tested to see if Salmonella was present. Prior to conducting a descriptive analysis with chi-square analysis in STATA, the raw data were arranged, coded, and entered into an Excel spreadsheet. From the 384 samples that were collected, 62 (16.15%) isolates were found; of these, 9.9%, 3.65, and 2.6% were discovered in feces, eggs, and meat, respectively. A statistically significant difference existed between the breeds (p-value=0.036). Salmonella infections were most prevalent in Brown bovans (32.83%), while least prevalent in Saso (30.81%). There was no statistically significant variation (p-value > 0.05) within each sample type, housing situation, and age group. 29 of the isolates (96.77%) had antimicrobial resistance. All isolates responded favorably to ampicillin and sulfamethoxazole-trimethoprim. Salmonella was found in a wide range of places, sample types, age groups, and breeds, indicating a more widespread distribution. Detection of Salmonella isolates indicated that it might be a developing problem for public health and poultry. Future studies ought to focus on isolating and identifying Salmonella from poultry raised in backyard systems and contrasting it with that raised in intensive farms, as well as molecular characterization for genetic studies and serotyping, as well as identifying the genes responsible for Salmonella pathogenicity and drug resistance.

Keywords: Bishoftu, Prevalence, Poultry, Resistance, Salmonella