Journal of
Yeast and Fungal Research

  • Abbreviation: J. Yeast Fungal Res.
  • Language: English
  • ISSN: 2141-2413
  • DOI: 10.5897/JYFR
  • Start Year: 2010
  • Published Articles: 129

Full Length Research Paper

Imaging flow cytometric analysis of Schizosaccharomyces pombe morphology

Radha Pyati1*, Lindsay L. Elvir1, C. Erica Charles1, Umawattee Seenath1, and Tom D. Wolkow2
1Department of Chemistry, University of North Florida, Jacksonville, FL 32224, USA. 2Department of Biology, University of Colorado at Colorado Springs, Colorado Springs, CO 80933, USA.
Email: [email protected]

  •  Accepted: 10 August 2011
  •  Published: 31 August 2011

Abstract

The morphology of Schizosaccharomyces pombe can be rapidly monitored in asynchronous, G2-rich populations using imaging flow cytometry (IFC). Cell morphology was analyzed in terms of length and aspect ratio before and after exposure to several toxins. The toxins target the DNA (hydroxyurea and phleomycin) or cytoskeletal elements (thiabendazole, carbendazim and latrunculin A) and exert well-characterized effects on morphology. Using IFC and yeast mutants, predictable morphological changes were detected accompanied with loss of gene products required during cellular responses to these toxins. IFC is a sensitive tool for accurate detection of subtle morphological changes in large, asynchronous S. pombe populations.

Key words: Imaging flow cytometry, fission yeast, Schizosaccharomyces pombe, cell cycle, morphology, genotoxin, cytoskeletal toxin.

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

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