Abstract

In Iraq, there is attempts to transfer the various industrial carbon waste to veterinary proteins depend on microorganisms by using of chemical process. Five different co-culture combinations (1:1 ratio, 1 × 10⁶ conidia) of Aspergillus niger and Trichoderma viride, mixing of A. niger and T. viride, in 24 and 48 h old monocultures of Aspergillussimilar mixing of A. in 24 and 48 h old monoculture of Trichoderma and the monocultures of both were evaluated for their potential performance of cellulases production. The study indicates that the cellulases obtained from compatible mixed cultures simultaneous mixing of both fungi have more enzyme activity as compared to their pure cultures and other combinations. The fermentation experiments were performed in solid stat fermentation (SSF). Incubation time, carbon sources and initial pH of fermentation medium was optimized with simultaneous mixed culture. It was revealed that the newspaper at pH = 5 and 40°C was the best source of carbon for the enhanced production of cellulase in the compatible mixed culture experiments after 8 days of incubation with 5.70 U/ml. Based on the reported results, it may be concluded that industrial carbon waste can be a potential substrate for production of cellulase, incorporation of co-culturing A. niger and T. viride. The aim of this work is to produce of Cellulase from waste paper and reduce the pollution.

Key words:  Aspergillus niger, Tricoderma viride, cellulase, culture.