Journal of
Yeast and Fungal Research

  • Abbreviation: J. Yeast Fungal Res.
  • Language: English
  • ISSN: 2141-2413
  • DOI: 10.5897/JYFR
  • Start Year: 2010
  • Published Articles: 125

Full Length Research Paper

Isolation of Aspergillus niger from three varieties of Bambara nuts for simultaneous production of phytase and tannase

H. V. Difo, E. Onyike, D. A. Ameh, G. C. Njoku and U. S. Ndidi*
Department of Biochemistry, Ahmadu Bello University, Zaria, Nigeria.
Email: [email protected]

  •  Accepted: 14 December 2012
  •  Published: 28 February 2013


The aim of this work was to isolate strain of Aspergillus niger for simultaneous production of phytase and tannase enzymes. In this study, A. niger strains were isolated from Bambara nuts (Vigna subterranea) of three different color seed coats. Growth of colonies in plates with tannic acid and phytic acid sodium salt-containing media indicate their ability to synthesize both enzymes. The strain of A. niger isolated from red color seed coat of Bambara nuts showed the highest potential for the production of both enzymes and was used to assay for phytase and tannase in three bean flours in solid-state fermentation.


Key words: Vigna subterranea, phytate, tannin, Aspergillus niger, colonies, solid-state fermentation, phytase, tannase.