Journal of
Yeast and Fungal Research

  • Abbreviation: J. Yeast Fungal Res.
  • Language: English
  • ISSN: 2141-2413
  • DOI: 10.5897/JYFR
  • Start Year: 2010
  • Published Articles: 126

Full Length Research Paper

Polycyclic aromatic hydrocarbons and small related molecules: Effects on Schizosaccharomyces pombe morphology measured by imaging flow cytometry

Tri Le
  • Tri Le
  • Department of Chemistry, University of North Florida, Jacksonville, FL 32224, U.S.A.
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Areeba Qureshi
  • Areeba Qureshi
  • Department of Chemistry, University of North Florida, Jacksonville, FL 32224, U.S.A.
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Joel Heisler
  • Joel Heisler
  • Department of Chemistry, University of North Florida, Jacksonville, FL 32224, U.S.A.
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Laura Bryant
  • Laura Bryant
  • Department of Chemistry, University of North Florida, Jacksonville, FL 32224, U.S.A.
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Jehan Shah
  • Jehan Shah
  • Department of Chemistry, University of North Florida, Jacksonville, FL 32224, U.S.A.
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Tom D. Wolkow
  • Tom D. Wolkow
  • Department of Biology, University of Colorado at Colorado Springs, Colorado Springs, Colorado, 80933, USA.
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Radha Pyati*
  • Radha Pyati*
  • Department of Chemistry, University of North Florida, Jacksonville, FL 32224, U.S.A.
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  •  Received: 30 May 2014
  •  Accepted: 06 August 2014
  •  Published: 11 August 2014

Abstract

Effects of polycyclic aromatic hydrocarbons and small related molecules on the morphology of fission yeast (Schizosaccharomyces pombe) are described. Polycyclic aromatic hydrocarbons are important environmental pollutants that act as carcinogens via several mechanisms of action. Fission yeast is a useful model organism for revealing the mechanisms by which these molecules affect the cell. None of the molecules studied affected cell length of wildtype or a rad26D mutant yeast relative to control, indicating that none of these operate like known genotoxins that lengthen the cell. Five compounds are shown to decrease cell width in wildtype fission yeast, but not in the rad26D strain. These results indicate that machinery controlling the cell’s width is affected by these molecules, and that this change is not detected when the rad26 protein is absent. These observations were made using imaging flow cytometry, which captures tens of thousands of two-dimensional cell images in a short time and provides statistically rigorous data on large cell populations.

 

Key words: Fission yeast, Schizosaccharomyces pombe, hydrocarbon, polycyclic aromatic hydrocarbon (PAH), morphology, imaging flow cytometry.