Journal of
Yeast and Fungal Research

  • Abbreviation: J. Yeast Fungal Res.
  • Language: English
  • ISSN: 2141-2413
  • DOI: 10.5897/JYFR
  • Start Year: 2010
  • Published Articles: 126

Full Length Research Paper

Effect of inoculum morphology on production of Nigerloxin by solid state fermentation

Vasantha, K. Y.
  • Vasantha, K. Y.
  • Fermentation Technology and Bio Engineering Department Central Food Technological Research Institute Mysore, 570020 India
  • Google Scholar
Saleem Javeed
  • Saleem Javeed
  • Fermentation Technology and Bio Engineering Department Central Food Technological Research Institute Mysore, 570020 India
  • Google Scholar
Chakradhar, D.
  • Chakradhar, D.
  • Fermentation Technology and Bio Engineering Department Central Food Technological Research Institute Mysore, 570020 India
  • Google Scholar
Sattur, A. P.*
  • Sattur, A. P.*
  • Fermentation Technology and Bio Engineering Department Central Food Technological Research Institute Mysore, 570020 India
  • Google Scholar


  •  Received: 16 October 2013
  •  Accepted: 28 January 2014
  •  Published: 31 May 2014

Abstract

Nigerloxin (2-amido-3-hydroxy-6-methoxy-5-methyle-4-(prop-1’enyl) benzoic acid) is a Lipoxygenase and Aldose reductase inhibitor produced by Aspergillus niger MTCC 5116. It is produced only under solid state fermentation (SSF) and the inoculum required for the inhibitor production is developed in submerged condition. As this is a newly discovered enzyme inhibitor with potential commercial success against diabetic complications, such as neuropathy and cataract, its complete fermentation process parametric study is not reported.  In this study, the role of physical parameters (spore suspen-sion, initial pH, incubation temperature and agitation) in spore germination and pellet size in inoculum development broth in submerged fermentation for the enhanced production of nigerloxin through SSF has been studied. It was concluded that 500 µl of spore suspension in the inoculum development broth at pH 7, incubated at 30°C and 200 rpm gave an ideal  pellet size 1.23 mm resulting in 6.0 mg of nigerloxin/g dry weight of wheat bran in SSF.

Key words: Nigerloxin, spore germination, solid state fermentation, Aspergillus niger.