Journal of
Yeast and Fungal Research

  • Abbreviation: J. Yeast Fungal Res.
  • Language: English
  • ISSN: 2141-2413
  • DOI: 10.5897/JYFR
  • Start Year: 2010
  • Published Articles: 126

Article in Press

Biochemical characterization of yeast species isolated from industry effluent for Azo dye decolorization

Birhanu Gizaw, Dinkitu Wako, Zerihun Tsgaye, Endegena Aynalem, Tesfaye Tamru

  •  Received: 12 June 2021
  •  Accepted: 08 October 2021
Azo dyes are the largest group organic dyes resistance to degradation and toxic to environment. Physico- chemical treatments are very expensive and releasing large amounts of secondary toxic degradant . Microbial treatments are optional alternatives. The main objective of this research is to isolate and characterize yeast biochemically from industry effluent for Azo dye degradation for bioremediation application. Screening and isolation of yeast was performed on YPDA media from industry effluent. Solid agar and broth assay test was performed for azo dye (Remazol yellow, Navy blue, Procion red MX-5B) decolorization. Yeast species was identified biochemically using BiologMicrostation depending on carbon assimilation and oxidation. The result revealed that the dominant yeast species from industrial effluent was Cryptococcus species(67.5%). Six yeast species was identified using biochemical characterization of BiologMicrostation where their probability is >75% and similarity index value >0.5.These are Yarrowla lipolytica (100%,0.7), Kluyveromyces delphensis (89%,0.64), Pichia mexicana (100%,0.62), Cryptococus albidus (99,0.58), Cryptococus albidus varalbidus (98%,0.65), Trichosporion begilli (100%,0.56). Three yeast species has below 75% probability and similarity index value (SIV) Cryptococcus luteolus (SIV,0.71), Issatchenkia scutuata var exigua (SIV,0.31), Rhodotrula acheniorum (SIV0.26).Trichosporion begilli has 92% degradation potential for Navy Blue azo dye in biologTurbidimeter. There for Trichosporion begilli could be a good candidate for mycoremediation service of Azodye treatment after toxicity analysis study.

Keywords: Azo dye, Bioremediation, Biochemical, Biolog, Microstation.