Journal of
Yeast and Fungal Research

  • Abbreviation: J. Yeast Fungal Res.
  • Language: English
  • ISSN: 2141-2413
  • DOI: 10.5897/JYFR
  • Start Year: 2010
  • Published Articles: 125

Table of Content: November 2010; 1(9)

November 2010

FLAG tag module for PCR based gene targeting

Epitope tagging of yeast proteins has become an efficient tool for biochemical analysis of protein of interest. The epitope-tagged proteins can be used for western blotting, immunoprecipitation and immunofluorescence experiments without the need to raise specific antibodies, thus saving considerable time and expense. We have constructed plasmid containing FLAG tag with kanMX6 module, which allows selection of...

Author(s): Rajeev Ranjan, Sumit Kumar Verma and Shakil Ahmed

November 2010

Identification of Candida glabrata and Candida parapsilosis strains by polymerase chain reaction assay using RPS0 gene fragment

Two Candida species were identified by the amplification of the RPS0 gene intron fragment. For this, two pairs of primers were used in PCR analysis performed with genomic DNA of clinical isolates of Candida. The primers designed are highly specific for their respective species and produce amplicons of the expected sizes and fail to amplify any DNA fragment from the other species tested. For Candida glabrata, the size of...

Author(s): Emira Noumi, Mejdi Snoussi, Maria del Pilar Vercher, Eulogio Valentin, Lucas Del Castillo and Amina Bakhrouf

November 2010

A specific protein A specific inhibitory protein to a restriction enzyme from Saccharomyces cerevisiae.

Bottled raphia palm wine was cultured in a laboratory medium amended with 0.5% acetic acid (pH 4.8). Two cultures of identified heat resistant yeast (HRY) and one unidentified yeast species were isolated. Cultures of the isolates grown on potato dextrose agar for 10 days at 26°C, survived pasteurization temperature at 80°C for 20 min and 85°C for 15 min. Of these HRY identified...

Author(s): Mukaram Shikara

November 2010

Determination of effective dose of garlic for controlling seedborne fungal disease of tomato.

Tomato seeds collected from farmer’s were treated with garlic tablet at concentration 1:3 w/v, 1:4 w/v, 1:5 w/v and 1:6 w/v for controlling seedborne fungal diseases. Seed health status of treated seeds was evaluated following blotter incubation method. Three different fungal pathogens viz., Aspergillus spp., Fusarium spp. and Penicillium spp. were identified from seed samples by blotter incubation method. Garlic...

Author(s): M. M. Rashid, A. B. M. Ruhul Amin and F. Rahman