Journal of
Yeast and Fungal Research

  • Abbreviation: J. Yeast Fungal Res.
  • Language: English
  • ISSN: 2141-2413
  • DOI: 10.5897/JYFR
  • Start Year: 2010
  • Published Articles: 125

Full Length Research Paper

Identification of Candida glabrata and Candida parapsilosis strains by polymerase chain reaction assay using RPS0 gene fragment

Emira Noumi1,2*, Mejdi Snoussi1,3, Maria del Pilar Vercher2, Eulogio Valentin2, Lucas Del Castillo2 and Amina Bakhrouf1
1Laboratoire d’Analyse, Traitement et Valorisation des Polluants de  l’Environnement et des Produits, Département de Microbiologie, Faculté de Pharmacie, Monastir, Tunisie. 2Departamento de Microbiología y Ecología, Facultad de Farmacia, Universidad de Valencia, Burjassot, Valencia, Spain. 3Laboratoire de Traitement et de Recyclage des Eaux. Centre de Recherches et des Technologies des eaux, Technopôle de Borj-Cédria, BP 901, 2050 Hammam-Lif, Tunisie.
Email: [email protected]

  •  Accepted: 02 November 2010
  •  Published: 30 November 2010

Abstract

Two Candida species were identified by the amplification of the RPS0 gene intron fragment. For this, two pairs of primers were used in PCR analysis performed with genomic DNA of clinical isolates of Candida. The primers designed are highly specific for their respective species and produce amplicons of the expected sizes and fail to amplify any DNA fragment from the other species tested. For Candida glabrata, the size of the amplicon was 406 and 150 bp for C. parapsilosis. The designed primers were able to amplify all C. glabrata isolates. One of three C. parapsilosis strains was confirmed as C. orthopsilosis, when we used the designed oligonucleotides. The used primers cannot amplify the other Candida species such as C. albicans. These results indicate that sequences of intron genes can be useful to specifically identify Candida strains by PCR. This molecular identification will be considered as an early identification of Candida species responsible for all candidiasis. 

Key words: Candida glabrata, C. parapsilosis, C. orthopsilosis, PCR- identification, RPS0 intron.