Research in Pharmaceutical Biotechnology

  • Abbreviation: Res. Pharm. Biotech.
  • Language: English
  • ISSN: 2141-2324
  • DOI: 10.5897/RPB
  • Start Year: 2009
  • Published Articles: 42

Full Length Research Paper

Evaluation of essential oils composition of methanolic Allium sativum extract on Trypanosoma brucei infected rats

Oluwatosin K. Yusuf
  • Oluwatosin K. Yusuf
  • Department of Biochemistry, Federal University of Technology, Trypanosomosis Research Unit, PMB 65, Minna, Nigeria.
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Clement O. Bewaji
  • Clement O. Bewaji
  • Department of Biochemistry, University of Ilorin, Ilorin, Nigeria.
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  • Article Number - 466C01B463
  • Vol.3(2), pp. 17-21, February 2011
  •  Accepted: 15 January 2011
  •  Published: 28 February 2011

Abstract

 

The essential oils composition and anti – trypanosomal activity of fermented methanolic Allium sativum extract was investigated. The crude extract was partially purified using column chromatography to give fractions A, B and C, which were further characterized by gas chromatographic – mass spectral (GC/MS) analysis. The fractions identified thirteen, sixteen and seventeen compounds respectively. The main components were oxygenated hydrocarbon (palmitoleic and steric acid) and n – hydrocarbon (unsaturated). The crude extract show anti- trypanosomal activity on Trypanosoma brucei infected rats.

 

Key words: Allium sativum, essential oils, gas chromatographic – mass spectral analysis, retention index (KI, anti- trypanosomal).