Scientific Research and Essays

  • Abbreviation: Sci. Res. Essays
  • Language: English
  • ISSN: 1992-2248
  • DOI: 10.5897/SRE
  • Start Year: 2006
  • Published Articles: 2752

Full Length Research Paper

Hepatitis B virus interacts with albumin precursor in vivo

Saeeda Baig1*, Anwar Ali Siddiqui 2, 3, Shamim Mushtaq3 and Jawed Shafqat4
  1Department of Biochemistry, Ziauddin Medical College, Ziauddin University, Karachi, Pakistan. 2Department of Biological and Biomedical Sciences, Research Juma Building, Aga Khan University Karachi, Pakistan. 3Aga Khan University Karachi, Pakistan. 4Department of Medical Biophysics and Biochemistry, Karolinska Institute, Stockholm, Sweden.
Email: [email protected], [email protected]

  •  Accepted: 23 September 2008
  •  Published: 31 October 2008

Abstract

 

A range of proteins has been recognized as mediators/hypothetical receptors for hepatitis B virus (HBV), but the results are conflicting and inconclusive especially regarding their biological significance. This study was aimed at identifying a novel HBV-interacting protein which would provide a better understanding of its transport in blood, attachment, fusion and entry into hepatocytes. Serum samples positive for HBV confirmed by PCR were subjected to ammonium sulfate fractionations at 50, 75, 100%, saturation. PCR of each fraction demonstrated amplification of HBV in 100% fraction. Protein analysis by SDS PAGE of fractions showed one band of approximately 69 kDa protein, in 100% fraction. The 100% fraction band was excised from gel and sequence was determined by MALDI TOF which showed mass values from 705 to 3722. Mass spectrometry of trypsinized 69 kDa species revealed peptide sequences that covered 54% of the serum albumin precursor amino acid sequence, with pI of 5.9. Western blot, carried out using primary anti-albumin precursor antibody, further validated this protein. This study establishes that Hepatitis B Virus binds to albumin precursor, suggesting its role in the initiation of HBV infection and hence may offer new therapeutic strategies against HBV infection.

 

Key words: Albumin precursor, hepatitis B virus, mass spectrometry, western blotting.